Evidence map›Paper›PMID 26456217›Full record

ArticleAnaerobe2015

Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola.

Xiaoping Xu, Bjorn Steffensen, Trista K Robichaud, Margarita Mikhailova, Veronica Lai, Ryan Montgomery, Lianrui Chu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Anaerobe, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xiaoping XuDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA. Electronic address: xux@uthscsa.edu.
Bjorn SteffensenDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA; Department of Periodontology, Tufts University School of Dental Medicine, Boston, MD, 02111, USA.
Trista K RobichaudDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
Margarita MikhailovaDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
Veronica LaiDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
Ryan MontgomeryDepartment of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
Lianrui ChuDepartment of Developmental Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
The University of Texas Health Science Center at San Antonio · USTufts University · US

Funding

Institutional Research Training GrantT32DE014318 · NIDCR · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Kenneth M Hargreaves · 2002 to 2026
$12.1M
MMP-2 and Fibronectin Fragmentation in Periodontal Diseases and Oral CancerR01DE017139 · NIDCR · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI STEFFENSEN, BJORN · 2006 to 2010
$1.7M
Fibronectin-binding Protein of Treponema Denticola in Periodontal DiseaseR21DE021520 · NIDCR · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI XU, XIAOPING · 2011 to 2012
$410k
INHIBITION OF CELL-MEDIATED PROMMP-2 ACTIVATION IN ORAL CANCERR03DE018135 · NIDCR · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI XU, XIAOPING · 2007 to 2008
$145k
NIDCR NIH HHS DE014318NIDCR NIH HHS DE017139NIDCR NIH HHS DE018135NIDCR NIH HHS DE021520NIDCR NIH HHS R01 DE017139NIDCR NIH HHS R03 DE018135NIDCR NIH HHS R21 DE021520NIDCR NIH HHS T32 DE014318
6 · The paper itself

Abstract

While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10(-7) M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells.

Indexed as

Bacterial ProteinsFibronectinsHumansKineticsProtein BindingTreponema denticolaTreponemal InfectionsVirulenceBacterial ProteinsFibronectinsFbpFibronectin binding proteinGene mutantPeriodontal diseaseTreponema denticola

Identifiers

PMID26456217
PMCPMC4663121
OpenAlexW1864800654

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.