Evidence map›Paper›PMID 26455965›Full record

ArticleMetabolism: clinical and experimental2015

Hepatic proteomic analysis revealed altered metabolic pathways in insulin resistant Akt1(+/-)/Akt2(-/-) mice.

Brian A Pedersen, Weiwen Wang, Jared F Taylor, Omar S Khattab, Yu-Han Chen, Robert A Edwards, Puya G Yazdi, Ping H Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolism: clinical and experimental, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Functional characterization of CD49fJournal of molecular histology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Brian A PedersenUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
Weiwen WangUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, Miami, FL, 33136.
Jared F TaylorUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
Omar S KhattabUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA.
Yu-Han ChenUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA.
Robert A EdwardsDepartment of Pathology, University of California at Irvine, Irvine, CA 92697, USA.
Puya G YazdiUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA.
Ping H WangUC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Department of Biological Chemistry, University of California at Irvine, Irvine, CA 92697, USA; Department of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA. Electronic address: phwang@uci.edu.
University of California, Irvine · USUniversity of Miami · US

Funding

Insulin's Control of Myocardial Mitochondria Bioenergetics and BiogenesisR01HL096987 · NHLBI · UNIVERSITY OF CALIFORNIA-IRVINE · PI WANG, PING H · 2010 to 2013
$1.5M
NHLBI NIH HHS R01 HL096987NHLBI NIH HHS R01-HL-096987
6 · The paper itself

Abstract

objectiveThe aim of this study was to identify liver proteome changes in a mouse model of severe insulin resistance and markedly decreased leptin levels.

methodsTwo-dimensional differential gel electrophoresis was utilized to identify liver proteome changes in AKT1(+/-)/AKT2(-/-) mice. Proteins with altered levels were identified with tandem mass spectrometry. Ingenuity Pathway Analysis was performed for the interpretation of the biological significance of the observed proteomic changes.

results11 proteins were identified from 2 biological replicates to be differentially expressed by a ratio of at least 1.3 between age-matched insulin resistant (Akt1(+/-)/Akt2(-/-)) and wild type mice. Albumin and mitochondrial ornithine aminotransferase were detected from multiple spots, which suggest post-translational modifications. Enzymes of the urea cycle were common members of top regulated pathways.

conclusionOur results help to unveil the regulation of the liver proteome underlying altered metabolism in an animal model of severe insulin resistance.

Indexed as

Insulin ResistanceAnimalsAnnexinsCarbonic AnhydrasesFemaleGlucoseLiverMaleMiceMice, Inbred C57BLOrnithine-Oxo-Acid TransaminaseProteomicsProto-Oncogene Proteins c-aktAkt1 protein, mouseAkt2 protein, mouseAnnexinsCarbonic AnhydrasesGlucoseOrnithine-Oxo-Acid TransaminaseProto-Oncogene Proteins c-aktAKTInsulin resistanceLiverMouseProteomics

Identifiers

PMID26455965
PMCPMC4641788
OpenAlexW1652365746

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.