Evidence map›Paper›PMID 26455557›Full record

ArticleJournal of structural biology2015

Automated batch fiducial-less tilt-series alignment in Appion using Protomo.

Alex J Noble, Scott M Stagg

Abstract read
In one paragraph

Article in Journal of structural biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Structure of the flotillin complex in a native membrane environment.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  5. Review
  6. The cryo-EM 3D image reconstruction of isolated Lethocerus indicus Z-discs.Journal of muscle research and cell motility · 2023
    Review
  7. Cryo-electron tomography to study viral infection.Biochemical Society transactions · 2023
    Review
  8. Quantitative prediction and measurement of Piezo's membrane footprint.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  9. Architecture of the human erythrocyte ankyrin-1 complex.Nature structural & molecular biology · 2022
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. The structure of natively iodinated bovine thyroglobulin.Acta crystallographica. Section D, Structural biology · 2021
    Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Alex J NobleDepartment of Physics, 77 Chieftan Way, Florida State University, Tallahassee, FL 32306, USA.
Scott M StaggDepartment of Chemistry and Biochemistry, 95 Chieftain Way, Florida State University, Tallahassee, FL 32306, USA; Institute of Molecular Biophysics, 91 Chieftan Way, Florida State University, Tallahassee, FL 32306, USA. Electronic address: sstagg@fsu.edu.

Funding

Tools For High-Throughput High-Resolution Three Dimensional Electron MicroscopyR01GM108753 · NIGMS · FLORIDA STATE UNIVERSITY · PI STAGG, SCOTT M · 2015 to 2020
$1.6M
The Structure and Assembly of the COPII CoatR01GM086892 · NIGMS · FLORIDA STATE UNIVERSITY · PI STAGG, SCOTT M · 2010 to 2014
$1.3M
Purchase of a direct electron camera for the Titan-Krios at FSUS10OD018142 · OD · FLORIDA STATE UNIVERSITY · PI TAYLOR, KENNETH ALLEN · 2014 to 2014
$384k
NIGMS NIH HHS GM086892NIGMS NIH HHS R01 GM086892NIGMS NIH HHS R01 GM108753NIH HHS S10 OD018142
6 · The paper itself

Abstract

The field of electron tomography has benefited greatly from manual and semi-automated approaches to marker-based tilt-series alignment that have allowed for the structural determination of multitudes of in situ cellular structures as well as macromolecular structures of individual protein complexes. The emergence of complementary metal-oxide semiconductor detectors capable of detecting individual electrons has enabled the collection of low dose, high contrast images, opening the door for reliable correlation-based tilt-series alignment. Here we present a set of automated, correlation-based tilt-series alignment, contrast transfer function (CTF) correction, and reconstruction workflows for use in conjunction with the Appion/Leginon package that are primarily targeted at automating structure determination with cryogenic electron microscopy.

Indexed as

DependovirusAutomation, LaboratoryCryoelectron MicroscopyElectron Microscope TomographyImage Processing, Computer-AssistedSoftwareAutomated processingCryo-electron tomographyMarker-free tilt-series alignmentProtomoSingle particle tomographyTransmission electron microscopy

Identifiers

PMID26455557
PMCPMC4633401

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.