ArticleJournal of structural biology2015
Automated batch fiducial-less tilt-series alignment in Appion using Protomo.
Article in Journal of structural biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed.
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- Structure of the flotillin complex in a native membrane environment.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- The cryo-EM 3D image reconstruction of isolated Lethocerus indicus Z-discs.Journal of muscle research and cell motility · 2023Review
- Cryo-electron tomography to study viral infection.Biochemical Society transactions · 2023Review
- Quantitative prediction and measurement of Piezo's membrane footprint.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Architecture of the human erythrocyte ankyrin-1 complex.Nature structural & molecular biology · 2022Article
- Waffle Method: A general and flexible approach for improving throughput in FIB-milling.Nature communications · 2022Article
- Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition.Nature communications · 2022Article
- Broadening access to cryoEM through centralized facilities.Trends in biochemical sciences · 2022Review
- ClusterAlign: A fiducial tracking and tilt series alignment tool for thick sample tomography.Biological imaging · 2022Article
- The structure of natively iodinated bovine thyroglobulin.Acta crystallographica. Section D, Structural biology · 2021Article
- Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress.Nature communications · 2021Article
- Coming of Age: Cryo-Electron Tomography as a Versatile Tool to Generate High-Resolution Structures at Cellular/Biological Interfaces.International journal of molecular sciences · 2021Review
- Current data processing strategies for cryo-electron tomography and subtomogram averaging.The Biochemical journal · 2021Review
- Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress.Research square · 2021Article
- Topaz-Denoise: general deep denoising models for cryoEM and cryoET.Nature communications · 2020Article
- Human parainfluenza virus fusion complex glycoproteins imaged in action on authentic viral surfaces.PLoS pathogens · 2020Article
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Authors and funding
2 authors.
Funding
Abstract
The field of electron tomography has benefited greatly from manual and semi-automated approaches to marker-based tilt-series alignment that have allowed for the structural determination of multitudes of in situ cellular structures as well as macromolecular structures of individual protein complexes. The emergence of complementary metal-oxide semiconductor detectors capable of detecting individual electrons has enabled the collection of low dose, high contrast images, opening the door for reliable correlation-based tilt-series alignment. Here we present a set of automated, correlation-based tilt-series alignment, contrast transfer function (CTF) correction, and reconstruction workflows for use in conjunction with the Appion/Leginon package that are primarily targeted at automating structure determination with cryogenic electron microscopy.
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