ArticleToxicology and applied pharmacology2015
Diacetyl and 2,3-pentanedione exposure of human cultured airway epithelial cells: Ion transport effects and metabolism of butter flavoring agents.
Article in Toxicology and applied pharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Toxicity, Chemistry, and Public Health Relevance of Emerging Nicotine Analog Vapes, Pods, and Pouches.Chemical research in toxicology · 2026Review
- Targeting integrin beta 4 in diacetyl-induced anoikis of the airway epithelium.Cell death discovery · 2026Article
- Single-cell resolution of human airway epithelial cells exposed to bronchiolitis obliterans-associated chemicals.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Odorant Metabolism in Humans.Angewandte Chemie (International ed. in English) · 2022Review
- Post-translational modifications to hemidesmosomes in human airway epithelial cells following diacetyl exposure.Scientific reports · 2022Article
- Implications of Electronic Cigarettes on the Safe Administration of Sedation and General Anesthesia in the Outpatient Dental Setting.Anesthesia progress · 2022Article
- Repetitive diacetyl vapor exposure promotes ubiquitin proteasome stress and precedes bronchiolitis obliterans pathology.Archives of toxicology · 2021Article
- More to Explore: Further Definition of Risk Factors for COPD - Differential Gender Difference, Modest Elevation in PMFrontiers in physiology · 2021Review
- Production of Plant-Associated Volatiles by Select Model and Industrially ImportantMicroorganisms · 2020Article
- Acute cytotoxicity and increased vascular endothelial growth factor after in vitro nitrogen mustard vapor exposure.Annals of the New York Academy of Sciences · 2020Article
- Airway basal cell injury after acute diacetyl (2,3-butanedione) vapor exposure.Toxicology letters · 2020Article
- Vaporized E-Cigarette Liquids Induce Ion Transport Dysfunction in Airway Epithelia.American journal of respiratory cell and molecular biology · 2019Article
- Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism.Scientific reports · 2019Article
- Transcriptomic response of primary human airway epithelial cells to flavoring chemicals in electronic cigarettes.Scientific reports · 2019Article
- Immunological and toxicological risk assessment of e-cigarettes.European respiratory review : an official journal of the European Respiratory Society · 2018Review
- Models of toxicity of diacetyl and alternative diones.Toxicology · 2017Review
- Diacetyl/l-Xylulose Reductase Mediates Chemical Redox Cycling in Lung Epithelial Cells.Chemical research in toxicology · 2017Article
- The Diacetyl-Exposed Human Airway Epithelial Secretome: New Insights into Flavoring-Induced Airways Disease.American journal of respiratory cell and molecular biology · 2017Article
- MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells.Scientific reports · 2017Article
- Proteomic Analysis of Primary Human Airway Epithelial Cells Exposed to the Respiratory Toxicant Diacetyl.Journal of proteome research · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Inhalation of butter flavoring by workers in the microwave popcorn industry may result in “popcorn workers' lung.” In previous in vivo studies rats exposed for 6 h to vapor from the flavoring agents, diacetyl and 2,3-pentanedione, acquired flavoring concentration-dependent damage of the upper airway epithelium and airway hyporeactivity to inhaled methacholine. Because ion transport is essential for lung fluid balance,we hypothesized that alterations in ion transport may be an early manifestation of butter flavoring-induced toxicity.We developed a system to expose cultured human bronchial/tracheal epithelial cells (NHBEs) to flavoring vapors. NHBEs were exposed for 6 h to diacetyl or 2,3-pentanedione vapors (25 or ≥ 60 ppm) and the effects on short circuit current and transepithelial resistance (Rt) were measured. Immediately after exposure to 25 ppm both flavorings reduced Na+ transport,without affecting Cl- transport or Na+,K+-pump activity. Rt was unaffected. Na+ transport recovered 18 h after exposure. Concentrations (100-360 ppm) of diacetyl and 2,3-pentanedione reported earlier to give rise in vivo to epithelial damage, and 60 ppm, caused death of NHBEs 0 h post-exposure. Analysis of the basolateral medium indicated that NHBEs metabolize diacetyl and 2,3-pentanedione to acetoin and 2-hydroxy-3-pentanone, respectively. The results indicate that ion transport is inhibited transiently in airway epithelial cells by lower concentrations of the flavorings than those that result in morphological changes of the cells in vivo or in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.