ArticleThe Journal of cell biology1989
An unusual beta-spectrin associated with clustered acetylcholine receptors.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 148 citations in OpenAlex.
- Periodic actin structures in neuronal axons are required to maintain microtubules.Molecular biology of the cell · 2017Article
- Flat clathrin lattices: stable features of the plasma membrane.Molecular biology of the cell · 2014Article
- Spectrin Breakdown Products (SBDPs) as Potential Biomarkers for Neurodegenerative Diseases.Current translational geriatrics and experimental gerontology reports · 2012Article
- Tropomodulins: pointed-end capping proteins that regulate actin filament architecture in diverse cell types.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Spectrin-based skeleton as an actor in cell signaling.Cellular and molecular life sciences : CMLS · 2012Review
- Cell organization, growth, and neural and cardiac development require αII-spectrin.Journal of cell science · 2011Article
- Characterization and expression of a heart-selective alternatively spliced variant of alpha II-spectrin, cardi+, during development in the rat.Journal of molecular and cellular cardiology · 2010Article
- Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila.The Journal of cell biology · 2010Article
- Targeted deletion of betaIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The actin binding domain of ACF7 binds directly to the tetratricopeptide repeat domains of rapsyn.Neuroscience · 2007Article
- A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.The Journal of cell biology · 2006Article
- On the functional interaction between nicotinic acetylcholine receptor and Na+,K+-ATPase.Pflugers Archiv : European journal of physiology · 2006Article
- Spectrins and ankyrinB constitute a specialized paranodal cytoskeleton.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Golgi localization of Syne-1.Molecular biology of the cell · 2003Article
- Neural cell adhesion molecule (NCAM) association with PKCbeta2 via betaI spectrin is implicated in NCAM-mediated neurite outgrowth.The Journal of cell biology · 2003Article
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- Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain.Molecular and cellular biology · 2002Article
- Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex.The Journal of cell biology · 2000Article
- Caenorhabditis elegans beta-G spectrin is dispensable for establishment of epithelial polarity, but essential for muscular and neuronal function.The Journal of cell biology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The clustering of acetylcholine receptors (AChR) in the postsynaptic membrane is an early event in the formation of the neuromuscular junction. The mechanism of clustering is still unknown, but is generally believed to be mediated by the postsynaptic cytoskeleton. We have identified an unusual isoform of beta-spectrin which colocalizes with AChR in AChR clusters isolated from rat myotubes in vitro. A related antigen is present postsynaptically at the neuromuscular junction of the rat. Immunoprecipitation, peptide mapping and immunofluorescence show that the beta-spectrin in AChR clusters resembles but is distinct from the beta-spectrin of human erythrocytes. alpha-Spectrin appears to be absent from AChR clusters. Semiquantitative immunofluorescence techniques indicate that there are from two to seven beta-spectrin molecules present for every clustered AChR, the higher values being obtained from rapidly prepared clusters, the lower values from clusters that require several minutes or more for isolation. Upon incubation of isolated AChR clusters for 1 h at room temperature, beta-spectrin is slowly depleted and the AChR redistribute into microaggregates. The beta-spectrin that remains associated with the myotube membrane is concentrated at these microaggregates. beta-Spectrin is quantitatively lost from clusters upon digestion with chymotrypsin, which causes AChR to redistribute in the plane of the membrane. These results suggest that AChR in clusters is closely linked to an unusual isoform of beta-spectrin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.