ArticleApplied and environmental microbiology2015
Premethylation of foreign DNA improves integrative transformation efficiency in Synechocystis sp. strain PCC 6803.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 29 citations in OpenAlex.
- Improving the Transformation Efficiency ofACS synthetic biology · 2025Article
- Methylation of foreign DNA overcomes the restriction barrier ofApplied and environmental microbiology · 2025Article
- Identification of the lipodepsipeptide selethramide encoded in a giant nonribosomal peptide synthetase from aProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review.Biotechnology letters · 2022Review
- A guanidine-degrading enzyme controls genomic stability of ethylene-producing cyanobacteria.Nature communications · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Function and Benefits of Natural Competence in Cyanobacteria: From Ecology to Targeted Manipulation.Life (Basel, Switzerland) · 2020Review
- Divergent methylation of CRISPR repeats and cas genes in a subtype I-D CRISPR-Cas-system.BMC microbiology · 2019Article
- Genomics Approaches to Deciphering Natural Transformation in Cyanobacteria.Frontiers in microbiology · 2019Article
- Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Restriction digestion of foreign DNA is one of the key biological barriers against genetic transformation in microorganisms. To establish a high-efficiency transformation protocol in the model cyanobacterium, Synechocystis sp. strain PCC 6803 (Synechocystis 6803), we investigated the effects of premethylation of foreign DNA on the integrative transformation of this strain. In this study, two type II methyltransferase-encoding genes, i.e., sll0729 (gene M) and slr0214 (gene C), were cloned from the chromosome of Synechocystis 6803 and expressed in Escherichia coli harboring an integration plasmid. After premethylation treatment in E. coli, the integration plasmid was extracted and used for transformation of Synechocystis 6803. The results showed that although expression of methyltransferase M had little impact on the transformation of Synechocystis 6803, expression of methyltransferase C resulted in 11- to 161-fold-higher efficiency in the subsequent integrative transformation of Synechocystis 6803. Effective expression of methyltransferase C, which could be achieved by optimizing the 5' untranslated region, was critical to efficient premethylation of the donor DNA and thus high transformation efficiency in Synechocystis 6803. Since premethylating foreign DNA prior to transforming Synechocystis avoids changing the host genetic background, the study thus provides an improved method for high-efficiency integrative transformation of Synechocystis 6803.
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