ArticleMicrobial cell factories2015
Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- A hybrid statistical-machine learning framework for optimization, prediction, and model-fitting of xylose and total sugar production from sugarcane bagasse using recombinant Escherichia coli-mediated hydrolysis.Archives of microbiology · 2026Article
- Advances, challenges, and future directions towards a cellulolytic Escherichia coli.Biotechnology for biofuels and bioproducts · 2026Review
- Techno-economic analysis of industrial-scale fermentation for formate dehydrogenase (FDH) production.Bioresources and bioprocessing · 2025Article
- Diversity and Bioprospection of Gram-positive Bacteria Derived from a Mayan Sinkhole.Microbial ecology · 2024Article
- Engineering of a newMetabolic engineering communications · 2021Article
- Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals.Microorganisms · 2021Review
- Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status.Molecular biotechnology · 2021Review
- Morphology engineering: a new strategy to construct microbial cell factories.World journal of microbiology & biotechnology · 2020Review
- Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4.BMC biotechnology · 2019Article
- Isolation of wheat bran-colonizing and metabolizing species from the human fecal microbiota.PeerJ · 2019Article
- An isolated cellulolyticBiotechnology for biofuels · 2017Article
- Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40.Asian-Australasian journal of animal sciences · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe microbial conversion of plant biomass into value added products is an attractive option to address the impacts of petroleum dependency. The Gram-negative bacterium Escherichia coli is commonly used as host for the industrial production of various chemical products with a variety of sugars as carbon sources. However, this strain neither produces endogenous cellulose degradation enzymes nor secrets heterologous cellulases for its poor secretory capacity. Thus, a cellulolytic E. coli strain capable of growth on plant biomass would be the first step towards producing chemicals and fuels. We previously identified the catalytic domain of a cellulase (Cel-CD) and its N-terminal sequence (N20) that can serve as carriers for the efficient extracellular production of target enzymes. This finding suggested that cellulose-utilizing E. coli can be engineered with minimal heterologous enzymes.
resultsIn this study, a β-glucosidase (Tfu0937) was fused to Cel-CD and its N-terminal sequence respectively to obtain E. coli strains that were able to hydrolyze the cellulose. Recombinant strains were confirmed to use the amorphous cellulose as well as cellobiose as the sole carbon source for growth. Furthermore, both strains were engineered with poly (3-hydroxybutyrate) (PHB) synthesis pathway to demonstrate the production of biodegradable polyesters directly from cellulose materials without exogenously added cellulases. The yield of PHB reached 2.57-8.23 wt% content of cell dry weight directly from amorphous cellulose/cellobiose. Moreover, we found the Cel-CD and N20 secretion system can also be used for the extracellular production of other hydrolytic enzymes.
conclusionsThis study suggested that a cellulose-utilizing E. coli was created based on a heterologous cellulase secretion system and can be used to produce biofuels and biochemicals directly from cellulose. This system also offers a platform for conversion of other abundant renewable biomass to biofuels and biorefinery products.
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