Evidence map›Paper›PMID 26452267›Full record

ArticlePloS one2015

Lack of Cytosolic Carboxypeptidase 1 Leads to Subfertility due to the Reduced Number of Antral Follicles in pcd3J-/- Females.

Ning Song, Nameun Kim, Rui Xiao, Hojun Choi, Hyo-Im Chun, Min-Hee Kang, Jin-Hoi Kim, Kunho Seo, Nagasundarapandian Soundrarajan, Jeong-Tae Do and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Tubulin post-translational modifications in meiosis.Seminars in cell & developmental biology · 2023
    Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Ning SongDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Nameun KimDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Rui XiaoDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Hojun ChoiDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Hyo-Im ChunDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Min-Hee KangDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Jin-Hoi KimDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Kunho SeoColleges of Veterinary Medicine, Konkuk University, Seoul, South Korea.
Nagasundarapandian SoundrarajanDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Jeong-Tae DoDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Hyuk SongDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Zhao-Jia GeCollege of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, P.R. China.
Chankyu ParkDepartment of Animal Biotechnology, Konkuk University, Seoul, South Korea.
Konkuk University · KRQingdao Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Females homozygous for the Purkinje cell degeneration mutation (pcd) are fertile, although the success rate is much lower than in the wild type. We performed detailed analysis of reproductive abnormalities of pcd females. The number of oocytes produced following exogenous gonadotropin treatment was much lower in pcd3J-/- females than in pcd3J+/+ females. Furthermore, the estrous cyclicity of pcd3J-/- females according to the appearance of the vagina was almost undetectable comparing to that of the wild type. Histological analyses and follicle counting of 4- and 8-week-old pcd3J-/- ovaries showed an increase in the number of secondary follicles and a decrease in the number of antral follicles, indicating that AGTPBP1/ CCP1 plays an important role in the development of secondary follicles into antral follicles. Consistent with a previous analysis of the pcd cerebellum, pcd3J-/- ovaries also showed a clear increase in the level of polyglutamylation. Gene expression analysis showed that both oocytes and cumulus cells express CCP1. However, Ccp4 and CCP6, which can compensate the function of CCP1, were not expressed in mouse ovaries. Failure of microtubule deglutamylation did not affect the structure and function of the meiotic spindle in properly aligning chromosomes in the center of the nucleus during meiosis in pcd3J-/- females. We also showed that the pituitary-derived growth and reproduction-related endocrine system functions normally in pcd3J-/- mice. The results of this study provide insight into additional functions of CCP1, which cannot be fully explained by the side chain deglutamylation of microtubules alone.

Indexed as

FertilityMeiosisAnimalsChromosomes, MammalianCumulus CellsFemaleGTP-Binding ProteinsMiceMice, KnockoutOocytesPurkinje CellsSerine-Type D-Ala-D-Ala CarboxypeptidaseSpindle ApparatusAgtpbp1 protein, mouseGTP-Binding ProteinsSerine-Type D-Ala-D-Ala Carboxypeptidase

Identifiers

PMID26452267
PMCPMC4599934
OpenAlexW2227082660

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.