Evidence map›Paper›PMID 26452215›Full record

ArticleNanoscale2015

Sandwich type plasmonic platform for MEF using silver fractals.

Sangram L Raut, Ryan Rich, Tanya Shtoyko, Ilkay Bora, Bo W Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanoscale, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sangram L RautDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX, USA. sraut@live.unthsc.edu and Department of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, USA. ignacy.gryczynski@unthsc.edu.
Ryan RichDepartment of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, USA. ignacy.gryczynski@unthsc.edu.
Tanya ShtoykoDepartment of Chemistry and Biochemistry, University of Texas at Tyler, Tyler, TX, USA.
Ilkay BoraDepartment of Chemistry, University of Copenhagen, Copenhagen, Denmark.
Bo W LaursenDepartment of Chemistry, University of Copenhagen, Copenhagen, Denmark.
Thomas Just SørensenDepartment of Chemistry, University of Copenhagen, Copenhagen, Denmark.
Julian BorejdoDepartment of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, USA. ignacy.gryczynski@unthsc.edu.
Zygmunt GryczynskiDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX, USA. sraut@live.unthsc.edu and Department of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, USA. ignacy.gryczynski@unthsc.edu.
Ignacy GryczynskiDepartment of Cell Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX, USA. ignacy.gryczynski@unthsc.edu.

Funding

Novel Fluorophores for Molecular and Cellular ImagingR01EB012003 · NIBIB · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI GRYCZYNSKI, ZYGMUNT, YU, YIHUA BRUCE · 2011 to 2014
$1.8M
Novel Approach to Image Mucin Release and SwellingR21EB017985 · NIBIB · TEXAS CHRISTIAN UNIVERSITY · PI GRYCZYNSKI, ZYGMUNT · 2014 to 2015
$342k
NIBIB NIH HHS R01 EB012003NIBIB NIH HHS R01EB12003NIBIB NIH HHS R21 EB017985NIBIB NIH HHS R21EB017985
6 · The paper itself

Abstract

In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N-methyl-azadioxatriangulenium chloride salt (Me-ADOTA·Cl) in PVA films made from 0.2% PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) were assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA·Cl in PVA film. In addition, we also tested ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than a 20-fold fluorescence enhancement (bright spots) accompanied by a decrease in the fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with or without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms.

Indexed as

FractalsFluorescent DyesHumansMicroscopy, ConfocalNanostructuresSerum AlbuminSilverFluorescent DyesSerum AlbuminSilver

Identifiers

PMID26452215
PMCPMC4808630

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.