ArticleACS synthetic biology2015
Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases.
Article in ACS synthetic biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
36 citing papers in PubMed, 97 citations in OpenAlex.
- Distinct repair outcomes from single and convergent replication fork collapse.Nature structural & molecular biology · 2026Article
- A recombineering-based platform for high-throughput genomic editing inApplied and environmental microbiology · 2025Article
- Development of a highly efficient base editing system for Lactobacilli to improve probiotics and dissect essential functions.Applied microbiology and biotechnology · 2025Article
- Recent advances in genome-scale engineering inEngineering microbiology · 2024Review
- Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing.Frontiers in genome editing · 2024Article
- CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions inFrontiers in bioengineering and biotechnology · 2024Article
- Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine.Genes & diseases · 2024Review
- Recent Advances in CRISPR-Cas Technologies for Synthetic Biology.Journal of microbiology (Seoul, Korea) · 2023Review
- The power and the promise of CRISPR/Cas9 genome editing for clinical application with gene therapy.Journal of advanced research · 2022Review
- Bacterial genome reductions: Tools, applications, and challenges.Frontiers in genome editing · 2022Review
- Review
- CRISPR-Based Genome Editing as a New Therapeutic Tool in Retinal Diseases.Molecular biotechnology · 2021Review
- Predictable control of RNA lifetime using engineered degradation-tuning RNAs.Nature chemical biology · 2021Article
- Genome Editing in Bacteria: CRISPR-Cas and Beyond.Microorganisms · 2021Review
- Portable CRISPR-Cas9Applied and environmental microbiology · 2021Article
- Comparative genome analysis of Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum decodes strain specific genes.PloS one · 2021Article
- CRISPR/Cas Technologies and Their Applications inFrontiers in bioengineering and biotechnology · 2021Review
- MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA.PLoS genetics · 2020Article
- Application of different types of CRISPR/Cas-based systems in bacteria.Microbial cell factories · 2020Review
- CRISPR-Cas9/CRISPRi tools for cell factory construction in E. coli.World journal of microbiology & biotechnology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Programmable CRISPR-Cas systems have augmented our ability to produce precise genome manipulations. Here we demonstrate and characterize the ability of CRISPR-Cas derived nickases to direct targeted recombination of both small and large genomic regions flanked by repetitive elements in Escherichia coli. While CRISPR directed double-stranded DNA breaks are highly lethal in many bacteria, we show that CRISPR-guided nickase systems can be programmed to make precise, nonlethal, single-stranded incisions in targeted genomic regions. This induces recombination events and leads to targeted deletion. We demonstrate that dual-targeted nicking enables deletion of 36 and 97 Kb of the genome. Furthermore, multiplex targeting enables deletion of 133 Kb, accounting for approximately 3% of the entire E. coli genome. This technology provides a framework for methods to manipulate bacterial genomes using CRISPR-nickase systems. We envision this system working synergistically with preexisting bacterial genome engineering methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.