Evidence map›Paper›PMID 26451488›Full record

ArticleCell2015

A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis.

Jixian Zhai, Sylvain Bischof, Haifeng Wang, Suhua Feng, Tzuu-Fen Lee, Chong Teng, Xinyuan Chen, Soo Young Park, Linshan Liu, Javier Gallego-Bartolome and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.

0numbers the graph read from it
0cells of the map it votes in
155citing papers in PubMed
50.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

155 citing papers in PubMed, 315 citations in OpenAlex.

  1. RNA regulation in plants.Science China. Life sciences · 2026
    Review
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  6. Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026
    Review
  7. Article
  8. Article
  9. Article
  10. A chromatin-linked CPL2-PHD2/3 module sustains multiple DNA methylation pathways and Polycomb silencing.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. DNA methylation in plant heterosis: mechanisms and prospects.Functional & integrative genomics · 2025
    Review
  12. RNA mNature plants · 2025
    Article
  13. DNA Methylation in Rice: Mechanisms, Regulatory Roles, and Beyond.International journal of molecular sciences · 2025
    Review
  14. Article
  15. Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025
    Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article

95 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Jixian ZhaiDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Sylvain BischofDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Haifeng WangHaixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou 350002, China; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Suhua FengDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Tzuu-Fen LeeDepartment of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
Chong TengDepartment of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
Xinyuan ChenDepartment of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Soo Young ParkDepartment of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Linshan LiuDepartment of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Javier Gallego-BartolomeDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA; Plant Biology Laboratory and Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Wanlu LiuDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Ian R HendersonDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
Blake C MeyersDepartment of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
Israel AusinHaixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: israelausin@fafu.edu.cn.
Steven E JacobsenDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jacobsen@ucla.edu.
University of California, Los Angeles · USBiotechnology Institute · USFujian Agriculture and Forestry University · CNHoward Hughes Medical Institute · USUniversity of Delaware · US

Funding

Genetics of DNA methylation patterning of arabidopsis.R37GM060398 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2009 to 2018
$3.0M
Genetics of DNA Methylation Patterning of ArabidopsisR01GM060398 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2000 to 2008
$2.2M
NIGMS NIH HHS GM60398NIGMS NIH HHS R01 GM060398NIGMS NIH HHS R37 GM060398
6 · The paper itself

Abstract

RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs (siRNAs) that target DNA methylation. Here, we demonstrate that Pol IV-dependent RNAs (P4RNAs) from wild-type Arabidopsis are surprisingly short in length (30 to 40 nt) and mirror 24-nt siRNAs in distribution, abundance, strand bias, and 5'-adenine preference. P4RNAs exhibit transcription start sites similar to Pol II products and are featured with 5'-monophosphates and 3'-misincorporated nucleotides. The 3'-misincorporation preferentially occurs at methylated cytosines on the template DNA strand, suggesting a co-transcriptional feedback to siRNA biogenesis by DNA methylation to reinforce silencing locally. These results highlight an unusual mechanism of Pol IV transcription and suggest a "one precursor, one siRNA" model for the biogenesis of 24-nt siRNAs in Arabidopsis.

Indexed as

AdenineArabidopsisDNA-Directed RNA PolymerasesDNA MethylationModels, BiologicalRNA, PlantRNA, Small InterferingTranscription Initiation SiteAdenineDNA-Directed RNA PolymerasesRNA, PlantRNA, Small Interfering

Identifiers

PMID26451488
PMCPMC5023148
OpenAlexW1900961661

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.