ArticleAdipocyte
Evaluation of reference genes for gene expression studies in human brown adipose tissue.
Article in Adipocyte. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Depot-specific metabolic and inflammatory profiles in perirenal and renal sinus adipose tissue.Molecular medicine (Cambridge, Mass.) · 2025Article
- Article
- LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions.International journal of molecular sciences · 2023Article
- RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue.Metabolites · 2022Article
- Transcriptome-based selection and validation of optimal reference genes in perirenal adipose developing of goat (Frontiers in veterinary science · 2022Article
- Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture.Scientific reports · 2020Article
- Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats.Animals : an open access journal from MDPI · 2020Article
- Genome-Wide Identification of RNA Editing Sites Affecting Intramuscular Fat in Pigs.Animals : an open access journal from MDPI · 2020Article
- Depot-specific UCP1 expression in human white adipose tissue and its association with obesity-related markers.International journal of obesity (2005) · 2020Article
- High-Fat Diet Alters the Expression of Reference Genes in Male Mice.Frontiers in nutrition · 2020Article
- Identification of reference genes and miRNAs for qRT-PCR in human esophageal squamous cell carcinoma.Medical oncology (Northwood, London, England) · 2017Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human brown adipose tissue (BAT) has during the last 5 year been subjected to an increasing research interest, due to its putative function as a target for future obesity treatments. The most commonly used method for molecular studies of human BAT is the quantitative polymerase chain reaction (qPCR). This method requires normalization to a reference gene (genes with uniform expression under different experimental conditions, e.g. similar expression levels between human BAT and WAT), but so far no evaluation of reference genes for human BAT has been performed. Two different microarray datasets with samples containing human BAT were used to search for genes with low variability in expression levels. Seven genes (FAM96B, GNB1, GNB2, HUWE1, PSMB2, RING1 and TPT1) identified by microarray analysis, and 8 commonly used reference genes (18S, B2M, GAPDH, LRP10, PPIA, RPLP0, UBC, and YWHAZ) were selected and further analyzed by quantitative PCR in both BAT containing perirenal adipose tissue and subcutaneous adipose tissue. Results were analyzed using 2 different algorithms (Normfinder and geNorm). Most of the commonly used reference genes displayed acceptably low variability (geNorm M-values <0.5) in the samples analyzed, but the novel reference genes identified by microarray displayed an even lower variability (M-values <0.25). Our data suggests that PSMB2, GNB2 and GNB1 are suitable novel reference genes for qPCR analysis of human BAT and we recommend that they are included in future gene expression studies of human BAT.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.