ArticleDevelopment, growth & differentiation2015
Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos.
Article in Development, growth & differentiation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- TEAD4 Regulates apical domain homeostasis and cell-positioning to maintain the trophectoderm lineage during mouse blastocyst development.Open biology · 2026Article
- aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo signaling kinase LATS1.Stem cell reports · 2026Article
- Geometric, cell cycle and maternal-to-zygotic transition-associated YAP dynamics during preimplantation embryo development.Developmental biology · 2025Article
- Generation of transient totipotent blastomere-like stem cells by short-term high-dose Pladienolide B treatment.Science China. Life sciences · 2025Article
- Divergent destinies: insights into the molecular mechanisms underlying EPI and PE fate determination.Life science alliance · 2025Review
- Geometric, cell cycle and maternal-to-zygotic transition-associated YAP dynamics during preimplantation embryo development.bioRxiv : the preprint server for biology · 2025Article
- Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells.eLife · 2024Article
- Tead4 and Tfap2c generate bipotency and a bistable switch in totipotent embryos to promote robust lineage diversification.Nature structural & molecular biology · 2024Article
- Origin and development of primary animal epithelia.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Article
- The aryl hydrocarbon receptor directs the differentiation of murine progenitor blastomeres.Cell biology and toxicology · 2023Article
- Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and mOpen biology · 2023Article
- The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization.Nature communications · 2023Article
- The role of polarization and early heterogeneities in the mammalian first cell fate decision.Current topics in developmental biology · 2023Review
- Journey of the mouse primitive endoderm: from specification to maturation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- The Regulation of the Hippo Pathway by Intercellular Junction Proteins.Life (Basel, Switzerland) · 2022Review
- Protein kinase Cα activation switches YAP1 from TEAD-mediated signaling to p73-mediated signaling.Cancer science · 2022Article
- Effects of palmitic acid on localization of embryo cell fate and blastocyst formation gene products.Reproduction (Cambridge, England) · 2022Article
- Emerging Role of Mechanical Forces in Cell Fate Acquisition.Frontiers in cell and developmental biology · 2022Review
- Chromatin as a sensor of metabolic changes during early development.Frontiers in cell and developmental biology · 2022Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In preimplantation mouse embryos, the Hippo signaling pathway plays a central role in regulating the fates of the trophectoderm (TE) and the inner cell mass (ICM). In early blastocysts with more than 32 cells, the Par-aPKC system controls polarization of the outer cells along the apicobasal axis, and cell polarity suppresses Hippo signaling. Inactivation of Hippo signaling promotes nuclear accumulation of a coactivator protein, Yap, leading to induction of TE-specific genes. However, whether similar mechanisms operate at earlier stages is not known. Here, we show that slightly different mechanisms operate in 16-cell stage embryos. Similar to 32-cell stage embryos, disruption of the Par-aPKC system activated Hippo signaling and suppressed nuclear Yap and Cdx2 expression in the outer cells. However, unlike 32-cell stage embryos, 16-cell stage embryos with a disrupted Par-aPKC system maintained apical localization of phosphorylated Ezrin/Radixin/Moesin (p-ERM), and the effects on Yap and Cdx2 were weak. Furthermore, normal 16-cell stage embryos often contained apolar cells in the outer position. In these cells, the Hippo pathway was strongly activated and Yap was excluded from the nuclei, thus resembling inner cells. Dissociated blastomeres of 8-cell stage embryos form polar-apolar couplets, which exhibit different levels of nuclear Yap, and the polar cell engulfed the apolar cell. These results suggest that cell polarization at the 16-cell stage is regulated by both Par-aPKC-dependent and -independent mechanisms. Asymmetric cell division is involved in cell polarity control, and cell polarity regulates cell positioning and most likely controls Hippo signaling.
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