ArticleExperimental eye research2016
Development of a novel in vivo corneal fibrosis model in the dog.
Article in Experimental eye research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- The molecular mechanisms of action of PPAR-γ agonists in the treatment of corneal alkali burns (Review).International journal of molecular medicine · 2016Pooled it
- Nintedanib Eye Drops Inhibit Alkali Burn-Induced Corneal Neovascularization Via RAP1/MEK/ERK Signaling Pathway.Investigative ophthalmology & visual science · 2026Article
- Transforming corneal alkali burn treatment: unveiling mechanisms and pioneering therapies from bench to bedside.Journal of translational medicine · 2025Review
- Angiogenesis, signaling pathways, and animal models.Chinese medical journal · 2025Review
- Comparative Histology of the Cornea and Palisades of Vogt in the Different Wild Ruminants (Bovidae, Camelidae, Cervidae, Giraffidae, Tragulidae).Animals : an open access journal from MDPI · 2022Article
- Corneal stromal repair and regeneration.Progress in retinal and eye research · 2022Review
- Time-dependent in situ structural and cellular aberrations in rabbit cornea in vivo after mustard gas exposure.Experimental eye research · 2022Article
- Concentration-associated pathology of alkali burn in a mouse model using anterior segment optical coherence tomography with angiography.Experimental eye research · 2022Article
- Decorin regulates collagen fibrillogenesis during corneal wound healing in mouse in vivo.Experimental eye research · 2022Article
- Evaluation of a novel combination of TRAM-34 and ascorbic acid for the treatment of corneal fibrosis in vivo.PloS one · 2022Article
- Recent Advances in Experimental Burn Models.Biology · 2021Review
- Ocular toxicity of mustard gas: A concise review.Toxicology letters · 2021Review
- Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders.Veterinary sciences · 2021Article
- Collagen matrix perturbations in corneal stroma of Ossabaw mini pigs with type 2 diabetes.Molecular vision · 2021Article
- A rabbit model for evaluating ocular damage from acrolein toxicity in vivo.Annals of the New York Academy of Sciences · 2020Article
- Recapitulation of normal collagen architecture in embryonic wounded corneas.Scientific reports · 2020Article
- Kinetics of Fluorescein in Tear Film After Eye Drop Instillation in Beagle Dogs: Does Size Really Matter?Frontiers in veterinary science · 2019Article
- A rabbit model of corneal Ectasia generated by treatment with collagenase type II.BMC ophthalmology · 2018Article
- Altering equine corneal fibroblast differentiation through Smad gene transfer.Veterinary ophthalmology · 2018Article
- Novel Combination BMP7 and HGF Gene Therapy Instigates Selective Myofibroblast Apoptosis and Reduces Corneal Haze In Vivo.Investigative ophthalmology & visual science · 2018Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The aim of this study was to develop a novel in vivo corneal model of fibrosis in dogs utilizing alkali burn and determine the ability of suberanilohydroxamic acid (SAHA) to inhibit corneal fibrosis using this large animal model. To accomplish this, we used seven research Beagle dogs. An axial corneal alkali burn in dogs was created using 1 N NaOH topically. Six dogs were randomly and equally assigned into 2 groups: A) vehicle (DMSO, 2 μL/mL); B) anti-fibrotic treatment (50 μM SAHA). The degree of corneal opacity, ocular health, and anti-fibrotic effects of SAHA were determined utilizing the Fantes grading scale, modified McDonald-Shadduck (mMS) scoring system, optical coherence tomography (OCT), corneal histopathology, immunohistochemistry (IHC), and transmission electron microscopy (TEM). The used alkali burn dose to produce corneal fibrosis was well tolerated as no significant difference in mMS scores between control and treatment groups (p = 0.89) were detected. The corneas of alkali burned dogs showed significantly greater levels of α-smooth muscle actin, the fibrotic marker, than the controls (p = 0.018). Total corneal thickness of all dogs post-burn was significantly greater than baseline OCT images irrespective of treatment (p = 0.004); TEM showed that alkali burned corneas had significantly greater minimum and maximum interfibrillar distances than the controls (p = 0.026, p = 0.018). The tested topical corneal alkali burn dose generated significant opacity and fibrosis in dog corneas without damaging the limbus as evidenced by histopathology, IHC, TEM, and OCT findings, and represents a viable large animal corneal fibrosis in vivo model. Additional in vivo SAHA dosing studies with larger sample size are warranted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.