Evidence map›Paper›PMID 26450592›Full record

ArticleJournal of insect science (Online)2015

Identification of immunity-related genes in the larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) by a next-generation sequencing-based transcriptome analysis.

Kyeongrin Bang, Sejung Hwang, Jiae Lee, Saeyoull Cho

Abstract read
In one paragraph

Article in Journal of insect science (Online), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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  5. Quality characteristics and protein digestibility ofJournal of animal science and technology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kyeongrin BangDepartment of Applied Biology, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University, Chuncheon, South Korea.
Sejung HwangDepartment of Applied Biology, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University, Chuncheon, South Korea.
Jiae LeeDepartment of Applied Biology, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University, Chuncheon, South Korea.
Saeyoull ChoDepartment of Applied Biology, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University, Chuncheon, South Korea saeyoullcho@kangwon.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To identify immune-related genes in the larvae of white-spotted flower chafers, next-generation sequencing was conducted with an Illumina HiSeq2000, resulting in 100 million cDNA reads with sequence information from over 10 billion base pairs (bp) and >50× transcriptome coverage. A subset of 77,336 contigs was created, and ∼35,532 sequences matched entries against the NCBI nonredundant database (cutoff, e < 10(-5)). Statistical analysis was performed on the 35,532 contigs. For profiling of the immune response, samples were analyzed by aligning 42 base sequence tags to the de novo reference assembly, comparing levels in immunized larvae to control levels of expression. Of the differentially expressed genes, 3,440 transcripts were upregulated and 3,590 transcripts were downregulated. Many of these genes were confirmed as immune-related genes such as pattern recognition proteins, immune-related signal transduction proteins, antimicrobial peptides, and cellular response proteins, by comparison to published data.

Indexed as

AnimalsColeopteraEscherichia coliGene Expression ProfilingHigh-Throughput Nucleotide SequencingInsect ProteinsLarvaSaccharomyces cerevisiaeSequence Analysis, RNAInsect ProteinsIlluminaimmuneProtaetia brevitarsis seulensisRNA-seqtranscription

Identifiers

PMID26450592
PMCPMC4626668

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.