ArticlePloS one2015
Acetylcholinesterases from the Disease Vectors Aedes aegypti and Anopheles gambiae: Functional Characterization and Comparisons with Vertebrate Orthologues.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- The patterns of acetylcholinesterases during developmental stages of Aedes aegypti and their susceptibility toward insecticides in egg stage.Scientific reports · 2026Article
- Potent and selective indole-based inhibitors targeting disease-transmitting mosquitoes.RSC medicinal chemistry · 2026Article
- The Molecular Properties of Honey Bee Acetylcholinesterase Reveal Opportunities to Avoid Off-Target Effects in Insecticide Discovery.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Enzyme Dynamics Determine the Potency and Selectivity of Inhibitors Targeting Disease-Transmitting Mosquitoes.ACS infectious diseases · 2024Article
- Larvicidal Activity of Constituents from the Main Brazilian Propolis Types: Green, Red, and Brown againstACS omega · 2024Article
- Uncovering the genetic diversity in Aedes aegypti insecticide resistance genes through global comparative genomics.Scientific reports · 2024Article
- Article
- Review
- Structure-Activity Relationships Reveal Beneficial Selectivity Profiles of Inhibitors Targeting Acetylcholinesterase of Disease-Transmitting Mosquitoes.Journal of medicinal chemistry · 2023Article
- Potential of Essential Oil-Based Anticholinesterase Insecticides againstMolecules (Basel, Switzerland) · 2022Review
- The in silico and in vitro analysis of donepezil derivatives for Anopheles acetylcholinesterase inhibition.PloS one · 2022Article
- Stop the crop: Insights into the insecticidal mode of action of cinnamodial against mosquitoes.Pesticide biochemistry and physiology · 2021Article
- Anopheles metabolic proteins in malaria transmission, prevention and control: a review.Parasites & vectors · 2020Review
- Cysteine-Targeted Insecticides againstACS medicinal chemistry letters · 2020Article
- Identification of Potential Inhibitors from Pyriproxyfen with Insecticidal Activity by Virtual Screening.Pharmaceuticals (Basel, Switzerland) · 2019Article
- Crystal structure of acetylcholinesterase catalytic subunits of the malaria vector Anopheles gambiae.Insect science · 2018Article
- Discovery of Species-selective and Resistance-breaking Anticholinesterase Insecticides for the Malaria Mosquito.Current medicinal chemistry · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mosquitoes of the Anopheles (An.) and Aedes (Ae.) genus are principal vectors of human diseases including malaria, dengue and yellow fever. Insecticide-based vector control is an established and important way of preventing transmission of such infections. Currently used insecticides can efficiently control mosquito populations, but there are growing concerns about emerging resistance, off-target toxicity and their ability to alter ecosystems. A potential target for the development of insecticides with reduced off-target toxicity is the cholinergic enzyme acetylcholinesterase (AChE). Herein, we report cloning, baculoviral expression and functional characterization of the wild-type AChE genes (ace-1) from An. gambiae and Ae. aegypti, including a naturally occurring insecticide-resistant (G119S) mutant of An. gambiae. Using enzymatic digestion and liquid chromatography-tandem mass spectrometry we found that the secreted proteins were post-translationally modified. The Michaelis-Menten constants and turnover numbers of the mosquito enzymes were lower than those of the orthologous AChEs from Mus musculus and Homo sapiens. We also found that the G119S substitution reduced the turnover rate of substrates and the potency of selected covalent inhibitors. Furthermore, non-covalent inhibitors were less sensitive to the G119S substitution and differentiate the mosquito enzymes from corresponding vertebrate enzymes. Our findings indicate that it may be possible to develop selective non-covalent inhibitors that effectively target both the wild-type and insecticide resistant mutants of mosquito AChE.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.