Evidence map›Paper›PMID 26447562›Full record

ArticleChemical research in toxicology2015

Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.

Jay P Patel, Mark L Sowers, Jason L Herring, Jacob A Theruvathu, Mark R Emmett, Bridget E Hawkins, Kangling Zhang, Douglas S DeWitt, Donald S Prough, Lawrence C Sowers

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical research in toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Spectroscopic and in vitro Investigations of FeChembiochem : a European journal of chemical biology · 2022
    Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jay P PatelDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Mark L SowersDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Jason L HerringDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Jacob A TheruvathuDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Mark R EmmettDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Bridget E HawkinsDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Kangling ZhangDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Douglas S DeWittDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Donald S ProughDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
Lawrence C SowersDepartment of Pharmacology and Toxicology, ‡Department of Biochemistry and Molecular Biology, §Department of Anesthesiology, ∥Department of Internal Medicine, Division of Hematology and Oncology, ⊥Moody Project for Translational Traumatic Brain Injury Research, Department of Anesthesiology, University of Texas Medical Branch , Galveston, Texas 77555, United States.
The University of Texas Medical Branch at Galveston · US

Funding

Molecular Mechanisms of Environmental InjuryT32ES007254 · NIEHS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI AMEREDES, BILL T · 1990 to 2022
$6.6M
Oxidation of 5-methylcytosine: DNA damage and epigenetic reprogrammingR01CA184097 · NCI · UNIVERSITY OF TEXAS MED BR GALVESTON · PI SOWERS, LAWRENCE C, ZHANG, KANGLING · 2014 to 2018
$1.6M
NCI NIH HHS CA184097NCI NIH HHS R01 CA184097NIEHS NIH HHS T32 ES007254NIEHS NIH HHS T32ES007254
6 · The paper itself

Abstract

The DNA of all organisms is metabolically active due to persistent endogenous DNA damage, repair, and enzyme-mediated base modification pathways important for epigenetic reprogramming and antibody diversity. The free bases released from DNA either spontaneously or by base excision repair pathways constitute DNA metabolites in living tissues. In this study, we have synthesized and characterized the stable-isotope standards for a series of pyrimidines derived from the normal DNA bases by oxidation and deamination. We have used these standards to measure free bases in small molecule extracts from rat brain. Free bases are observed in extracts, consistent with both endogenous DNA damage and 5-methylcytosine demethylation pathways. The most abundant free base observed is uracil, and the potential sources of uracil are discussed. The free bases measured in tissue extracts constitute the end product of DNA metabolism and could be used to reveal metabolic disturbances in human disease.

Indexed as

Brain ChemistryDNA DamageAnimalsBrainChromatography, High Pressure LiquidGas Chromatography-Mass SpectrometryMagnetic Resonance SpectroscopyMolecular StructureOxidation-ReductionPyrimidinesRatsPyrimidines

Identifiers

PMID26447562
PMCPMC7986959
OpenAlexW2277886745

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.