ArticleBioMed research international2015
A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Prevalence of Zoonotic Parasites and Genetic Diversity ofJournal of parasitology research · 2026Article
- Development of an colloidal gold immunochromatography assay strip for the diagnosis ofFrontiers in veterinary science · 2025Article
- A Multiplex PCR Assay for Simultaneous Detection ofVeterinary sciences · 2024Article
- Development of a New LAMP Assay for the Detection ofFrontiers in veterinary science · 2021Article
- Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms.The Korean journal of parasitology · 2019Article
- Screening of Zoonotic Parasites in Playground Sandboxes of Public Parks from Subtropical Mexico.Journal of parasitology research · 2019Article
- Article
- A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool.PLoS neglected tropical diseases · 2017Article
- Molecular Diagnostics for Soil-Transmitted Helminths.The American journal of tropical medicine and hygiene · 2016Review
- Molecular diagnosis of infectious parasites in the post-COVID-19 era.Tropical parasitologyArticle
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ancylostoma ceylanicum, A. caninum, and Giardia lamblia assemblage A are common intestinal parasites of dogs and cats; they can also infect humans, causing parasitic zoonoses. In this study, a multiplex PCR method was developed for simultaneous identification and detection of those three zoonotic parasites. Three pairs of specific primers were designed based on ITS sequence of A. ceylanicum and A. caninum and TPI gene of G. lamblia available in the GenBank. The multiplex PCR reaction system was established by optimizing the reaction condition, and a series of tests on the sensitivity, specificity, and clinical application were also conducted. Results showed that three target fragments were amplified specifically; the detection limit was 10 eggs for both A. ceylanicum and A. caninum, 72 pg DNA for G. lamblia. Of 112 clinical fecal samples, 34.8% and 17.8% samples were positive for A. caninum and A. ceylanicum, respectively, while only 2.7% samples were positive for G. lamblia assemblage A. It is concluded that the established multiplex PCR assay is a convenient, rapid, cost-effective, and high-efficiency method for molecular detection and epidemiological investigation of three zoonotic parasites.
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