ArticleBMC biotechnology2015
Development of an efficient RNA interference method by feeding for the microcrustacean Daphnia.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.Development (Cambridge, England) · 2026Article
- Effects of RNAi-by-feeding on DNA methyltransferases inEnvironmental epigenetics · 2026Article
- Evaluation of dsRNA produced inFrontiers in insect science · 2026Article
- Maternal effects in the model system Daphnia: the ecological past meets the epigenetic future.Heredity · 2025Review
- Screening of morphology-related genes based on predator-induced transcriptome sequencing and the functional analysis ofCurrent zoology · 2024Article
- Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis.Frontiers in zoology · 2023Article
- Review
- RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation inInternational journal of molecular sciences · 2021Article
- Article
- RNAi-Based Biocontrol Products: Market Status, Regulatory Aspects, and Risk Assessment.Frontiers in insect science · 2021Article
- Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes.Insect biochemistry and molecular biology · 2020Article
- RNA Interference by Ingested dsRNA-Expressing Bacteria to Study Shell Biosynthesis and Pigmentation in Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2019Article
- RNA interference may result in unexpected phenotypes in Caenorhabditis elegans.Nucleic acids research · 2019Article
- Hemoglobin Levels Modulate Nitrite Toxicity to Daphnia magna.Scientific reports · 2018Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2017Article
- A New Reference Genome Assembly for the MicrocrustaceanG3 (Bethesda, Md.) · 2017Article
- Population Genomics ofGenetics · 2017Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundRNA interference (RNAi) is an important molecular tool for analysis of gene function in vivo. Daphnia, a freshwater microcrustacean, is an emerging model organism for studying cellular and molecular processes involved in aging, development, and ecotoxicology especially in the context of environmental variation. However, in spite of the availability of a fully sequenced genome of Daphnia pulex, meaningful mechanistic studies have been hampered by a lack of molecular techniques to alter gene expression. A microinjection method for gene knockdown by RNAi has been described but the need for highly specialized equipment as well as technical expertise limits the wider application of this technique. In addition to being expensive and technically challenging, microinjections can only target genes expressed during embryonic stages, thus making it difficult to achieve effective RNAi in adult organisms.
resultsIn our present study we present a bacterial feeding method for RNAi in Daphnia. We used a melanic Daphnia species (Daphnia melanica) that exhibits dark pigmentation to target phenoloxidase, a key enzyme in the biosynthesis of melanin. We demonstrate that our RNAi method results in a striking phenotype and that the phenoloxidase mRNA expression and melanin content, as well as survival following UV insults, are diminished as a result of RNAi.
conclusionsOverall, our results establish a new method for RNAi in Daphnia that significantly advances further use of Daphnia as a model organism for functional genomics studies. The method we describe is relatively simple and widely applicable for knockdown of a variety of genes in adult organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.