ArticleScientific reports2015
Real-time imaging of hydrogen peroxide dynamics in vegetative and pathogenic hyphae of Fusarium graminearum.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Exploitation of Host-Derived N-Feruloylputrescine by Fusarium graminearum Promotes DON Biosynthesis and Wheat Rachis Colonisation.Molecular plant pathology · 2026Article
- Heme sensing and trafficking in fungi.Fungal biology reviews · 2023Article
- Reactive oxidant species induced by antifungal drugs: identity, origins, functions, and connection to stress-induced cell death.Frontiers in cellular and infection microbiology · 2023Review
- NADPH Oxidase Gene,Frontiers in microbiology · 2022Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- The spatiotemporal control of KatG2 catalase-peroxidase contributes to the invasiveness of Fusarium graminearum in host plants.Molecular plant pathology · 2019Article
- Redox signalling from NADPH oxidase targets metabolic enzymes and developmental proteins in Fusarium graminearum.Molecular plant pathology · 2019Article
- Pathways of Pathogenicity: Transcriptional Stages of Germination in the Fatal Fungal PathogenmSphere · 2018Article
- The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum.Molecular plant pathology · 2018Article
- Optimization of the HyPer sensor for robust real-time detection of hydrogen peroxide in the rice blast fungus.Molecular plant pathology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Balanced dynamics of reactive oxygen species in the phytopathogenic fungus Fusarium graminearum play key roles for development and infection. To monitor those dynamics, ratiometric analysis using the novel hydrogen peroxide (H2O2) sensitive fluorescent indicator protein HyPer-2 was established for the first time in phytopathogenic fungi. H2O2 changes the excitation spectrum of HyPer-2 with an excitation maximum at 405 nm for the reduced and 488 nm for the oxidized state, facilitating ratiometric readouts with maximum emission at 516 nm. HyPer-2 analyses were performed using a microtiter fluorometer and confocal laser scanning microscopy (CLSM). Addition of external H2O2 to mycelia caused a steep and transient increase in fluorescence excited at 488 nm. This can be reversed by the addition of the reducing agent dithiothreitol. HyPer-2 in F. graminearum is highly sensitive and specific to H2O2 even in tiny amounts. Hyperosmotic treatment elicited a transient internal H2O2 burst. Hence, HyPer-2 is suitable to monitor the intracellular redox balance. Using CLSM, developmental processes like nuclear division, tip growth, septation, and infection structure development were analyzed. The latter two processes imply marked accumulations of intracellular H2O2. Taken together, HyPer-2 is a valuable and reliable tool for the analysis of environmental conditions, cellular development, and pathogenicity.
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