ArticleScientific reports2015
Stacked thin layers of metaphase chromatin explain the geometry of chromosome rearrangements and banding.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Hypothesis: The opposing pulling forces exerted by spindle microtubules can cause sliding of chromatin layers and facilitate sister chromatid resolution.Frontiers in genetics · 2023Article
- Differentially accessible, single copy sequences form contiguous domains along metaphase chromosomes that are conserved among multiple tissues.Molecular cytogenetics · 2021Article
- Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Scaling Laws for Mitotic Chromosomes.Frontiers in cell and developmental biology · 2021Article
- Mitotic chromosome organization: General rules meet species-specific variability.Computational and structural biotechnology journal · 2020Review
- Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.The EMBO journal · 2019Article
- Chromatin Higher-Order Folding: A Perspective with Linker DNA Angles.Biophysical journal · 2018Review
- Linking Chromatin Fibers to Gene Folding by Hierarchical Looping.Biophysical journal · 2017Review
- Genome maintenance in the context of 4D chromatin condensation.Cellular and molecular life sciences : CMLS · 2016Review
- Hierarchical looping of zigzag nucleosome chains in metaphase chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Novel insights into mitotic chromosome condensation.F1000Research · 2016Review
- Bridging chromatin structure and function over a range of experimental spatial and temporal scales by molecular modeling.Wiley interdisciplinary reviews. Computational molecular scienceArticle
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1 author at 1 institution in 1 country.
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Abstract
The three-dimensional organization of tightly condensed chromatin within metaphase chromosomes has been one of the most challenging problems in structural biology since the discovery of the nucleosome. This study shows that chromosome images obtained from typical banded karyotypes and from different multicolour cytogenetic analyses can be used to gain information about the internal structure of chromosomes. Chromatin bands and the connection surfaces in sister chromatid exchanges and in cancer translocations are planar and orthogonal to the chromosome axis. Chromosome stretching produces band splitting and even the thinnest bands are orthogonal and well defined, indicating that short stretches of DNA can occupy completely the chromosome cross-section. These observations impose strong physical constraints on models that attempt to explain chromatin folding in chromosomes. The thin-plate model, which consists of many stacked layers of planar chromatin perpendicular to the chromosome axis, is compatible with the observed orientation of bands, with the existence of thin bands, and with band splitting; it is also compatible with the orthogonal orientation and planar geometry of the connection surfaces in chromosome rearrangements. The results obtained provide a consistent interpretation of the chromosome structural properties that are used in clinical cytogenetics for the diagnosis of hereditary diseases and cancers.
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