Evidence map›Paper›PMID 26445444›Full record

ArticleBiophysical journal2015

Thermal Motion of DNA in an MspA Pore.

Bo Lu, Stephen Fleming, Tamas Szalay, Jene Golovchenko

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Bo LuDepartment of Physics, Harvard University, Cambridge, Massachusetts.
Stephen FlemingDepartment of Physics, Harvard University, Cambridge, Massachusetts.
Tamas SzalaySchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
Jene GolovchenkoDepartment of Physics, Harvard University, Cambridge, Massachusetts; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts. Electronic address: golovchenko@physics.harvard.edu.
Harvard University · US

Funding

Electronic Sequencing in NanoporesR01HG003703 · NHGRI · HARVARD UNIVERSITY · PI BRANTON, DANIEL NONE, GOLOVCHENKO, JENE A · 2005 to 2014
$16.1M
NHGRI NIH HHS HG003703NHGRI NIH HHS R01 HG003703
6 · The paper itself

Abstract

We report on an experiment and calculations that determine the thermal motion of a voltage-clamped single-stranded DNA-NeutrAvidin complex in a Mycobacterium smegmatis porin A nanopore. The electric force and diffusion constant of DNA inside a Mycobacterium smegmatis porin A pore were determined to evaluate the thermal position fluctuations of DNA. We show that an out-of-equilibrium state returns to equilibrium so quickly that experiments usually measure a weighted average over the equilibrium position distribution. Averaging over the equilibrium position distribution is consistent with results of state-of-the-art nanopore sequencing experiments. It is shown how a reduction in thermal position fluctuations can be achieved by increasing the electrophoretic force used in nanopore sequencing devices.

Indexed as

MotionTemperatureAvidinDiffusionDNA, Single-StrandedModels, GeneticModels, MolecularMycobacterium smegmatisNanoporesPatch-Clamp TechniquesPorinsAvidinDNA, Single-StrandedmspA protein, Mycobacterium smegmatisneutravidinPorins

Identifiers

PMID26445444
PMCPMC4601093
OpenAlexW1834211884

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.