ArticleBiophysical journal2015
Rapid Bioinformatic Identification of Thermostabilizing Mutations.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- High-resolution structures of human NHE6 and NHE9 elucidate endosomal ion and lipid interactions.Nature communications · 2026Article
- Genomic and evolutionary factors influencing the prediction accuracy of optimal growth temperature in prokaryotes.mSystems · 2026Article
- A unified compendium of prokaryotic and viral genomes from over 300 anaerobic digestion microbiomes.Environmental microbiome · 2024Article
- A social niche breadth score reveals niche range strategies of generalists and specialists.Nature ecology & evolution · 2023Article
- Polymeric carbohydrates utilization separates microbiomes into niches: insights into the diversity of microbial carbohydrate-active enzymes in the inner shelf of the Pearl River Estuary, China.Frontiers in microbiology · 2023Article
- A Thermodynamic Atlas of Proteomes Reveals Energetic Innovation across the Tree of Life.Molecular biology and evolution · 2022Article
- IMPROvER: the Integral Membrane Protein Stability Selector.Scientific reports · 2020Article
- Theoretical identification of thermostabilizing amino acid mutations for G-protein-coupled receptors.Biophysical reviews · 2020Review
- TEMPURA: Database of Growth TEMPeratures of Usual and RAre Prokaryotes.Microbes and environments · 2020Article
- Molecular simulation of PcCel45A protein expressed from Aspergillus nidulans to understand its structure, dynamics, and thermostability.Journal of molecular modeling · 2019Article
- Predicting the optimal growth temperatures of prokaryotes using only genome derived features.Bioinformatics (Oxford, England) · 2019Article
- Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- Identifying Thermostabilizing Mutations in Membrane Proteins by Bioinformatics.Biophysical journal · 2015Article
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4 authors.
Funding
Abstract
Ex vivo stability is a valuable protein characteristic but is laborious to improve experimentally. In addition to biopharmaceutical and industrial applications, stable protein is important for biochemical and structural studies. Taking advantage of the large number of available genomic sequences and growth temperature data, we present two bioinformatic methods to identify a limited set of amino acids or positions that likely underlie thermostability. Because these methods allow thousands of homologs to be examined in silico, they have the advantage of providing both speed and statistical power. Using these methods, we introduced, via mutation, amino acids from thermoadapted homologs into an exemplar mesophilic membrane protein, and demonstrated significantly increased thermostability while preserving protein activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.