Evidence map›Paper›PMID 26444999›Full record

ArticlePloS one2015

Geometrical versus Random β-TCP Scaffolds: Exploring the Effects on Schwann Cell Growth and Behavior.

Lauren Sweet, Yunqing Kang, Christopher Czisch, Lukasz Witek, Yang Shi, Jim Smay, Giles W Plant, Yunzhi Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Article
  6. 3D Printing for Bone Regeneration.Current osteoporosis reports · 2020
    Review
  7. Form and functional repair of long bone using 3D-printed bioactive scaffolds.Journal of tissue engineering and regenerative medicine · 2018
    Article
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Lauren SweetDepartment of Orthopaedic Surgery, Stanford University, Stanford, California, United States of America; Department of Biology, Stanford University, Stanford, California, United States of America.
Yunqing KangDepartment of Orthopaedic Surgery, Stanford University, Stanford, California, United States of America; Department of Ocean and Mechanical Engineering, Florida Atlantic University, Boca Raton, Florida, United States of America.
Christopher CzischDepartment of Neurosurgery, Stanford University, Stanford, California, United States of America.
Lukasz WitekSchool of Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma, United States of America.
Yang ShiSchool of Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma, United States of America.
Jim SmaySchool of Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma, United States of America.
Giles W PlantDepartment of Neurosurgery, Stanford University, Stanford, California, United States of America.
Yunzhi YangDepartment of Orthopaedic Surgery, Stanford University, Stanford, California, United States of America; Department of Materials Science and Engineering, Stanford University, Stanford, California, United States of America; Department of Bioengineering, Stanford University, Stanford, California, United States of America.
Stanford University · USOklahoma State University · US

Funding

Microengineered Osteons for Bone Tissue EngineeringR01AR057837 · NIAMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI YANG, YUNZHI · 2010 to 2018
$5.1M
Engineering Vascularized Bone Tissues By Microfabrication And ScaffoldingR01DE021468 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI YANG, YUNZHI · 2011 to 2014
$1.8M
NIAMS NIH HHS R01 AR057837NIAMS NIH HHS R01AR057837NIDCR NIH HHS R01 DE021468NIDCR NIH HHS R01DE021468
6 · The paper itself

Abstract

Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.

Indexed as

AnimalsBecaplerminBiocompatible MaterialsBone RegenerationCalcium PhosphatesCell ProliferationGene ExpressionNeovascularization, PhysiologicNeurotrophin 3PorosityProto-Oncogene Proteins c-sisRatsRats, Inbred F344Schwann CellsTissue ScaffoldsVascular Endothelial Growth Factor ABecaplerminbeta-tricalcium phosphateBiocompatible MaterialsCalcium PhosphatesNeurotrophin 3Proto-Oncogene Proteins c-sisVascular Endothelial Growth Factor A

Identifiers

PMID26444999
PMCPMC4596809
OpenAlexW1924961610

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.