ArticleVirology journal2015
Human papillomavirus genotyping by Linear Array and Next-Generation Sequencing in cervical samples from Western Mexico.
Article in Virology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Oral Health-Head and Neck Cancers: Addressing Confounding Through Negative Control and Quantitative Bias Analyses.Community dentistry and oral epidemiology · 2026Article
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- Unraveling the Complex Nexus of Human Papillomavirus (HPV) in Extragenital Keratinocyte Skin Tumors: A Comprehensive Analysis of Bowen's Disease and In Situ Squamous-Cell Carcinoma.Journal of clinical medicine · 2024Article
- Article
- Development of a simplified and cost-effective sample preparation method for genotyping of human papillomavirus by next-generation sequencing.Archives of virology · 2023Article
- Prevalence and distribution of selected cervical human papillomavirus types in HIV infected and HIV uninfected women in South Africa, 1989-2021: A narrative review.Southern African journal of infectious diseases · 2022Review
- The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland.Diagnostics (Basel, Switzerland) · 2021Article
- Human Papillomavirus Oral Infection: Review of Methodological Aspects and Epidemiology.Pathogens (Basel, Switzerland) · 2021Review
- Analysis of HPV Integrations in Mexican Pre-Tumoral Cervical Lesions Reveal Centromere-Enriched Breakpoints and Abundant Unspecific HPV Regions.International journal of molecular sciences · 2021Article
- NanoString Technology for Human Papillomavirus Typing.Viruses · 2021Article
- High frequency of HPV genotypes 59, 66, 52, 51, 39 and 56 in women from Western Mexico.BMC infectious diseases · 2020Article
- Detection of Alpha, Beta, Gamma, and Unclassified Human Papillomaviruses in Cervical Cancer Samples From Mexican Women.Frontiers in cellular and infection microbiology · 2020Article
- Identification by high-throughput sequencing of HPV variants and quasispecies that are untypeable by linear reverse blotting assay in cervical specimens.Papillomavirus research (Amsterdam, Netherlands) · 2019Article
- Cross-hybridization between HPV genotypes in the Linear Array Genotyping Test confirmed by Next-Generation Sequencing.Diagnostic pathology · 2019Article
- E6/E7 and E6Frontiers in immunology · 2019Article
- Quantitative DNA methylation analysis of paired box gene 1 and LIM homeobox transcription factor 1 α genes in cervical cancer.Oncology letters · 2018Article
- Genotyping of high-risk anal human papillomavirus (HPV): ion torrent-next generation sequencing vs. linear array.Virology journal · 2017Article
- Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment.Journal of clinical microbiology · 2017Article
- Genetic variability in E6, E7 and L1 genes of Human Papillomavirus 62 and its prevalence in Mexico.Infectious agents and cancer · 2017Article
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Authors and funding
11 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Linear Array® (LA) genotyping test is one of the most used methodologies for Human papillomavirus (HPV) genotyping, in that it is able to detect 37 HPV genotypes and co-infections in the same sample. However, the assay is limited to a restricted number of HPV, and sequence variations in the detection region of the HPV probes could give false negatives results. Recently, 454 Next-Generation sequencing (NGS) technology has been efficiently used also for HPV genotyping; this methodology is based on massive sequencing of HPV fragments and is expected to be highly specific and sensitive. In this work, we studied HPV prevalence in cervixes of women in Western Mexico by LA and confirmed the genotypes found by NGS.
methodsTwo hundred thirty three cervical samples from women Without cervical lesions (WCL, n = 48), with Cervical intraepithelial neoplasia grade 1 (CIN I, n = 98), or with Cervical cancer (CC, n = 87) were recruited, DNA was extracted, and HPV positivity was determined by PCR amplification using PGMY09/11 primers. All HPV- positive samples were genotyped individually by LA. Additionally, pools of amplicons from the PGMY-PCR products were sequenced using 454 NGS technology. Results obtained by NGS were compared with those of LA for each group of samples.
resultsWe identified 35 HPV genotypes, among which 30 were identified by both technologies; in addition, the HPV genotypes 32, 44, 74, 102 and 114 were detected by NGS. These latter genotypes, to our knowledge, have not been previously reported in Mexican population. Furthermore, we found that LA did not detect, in some diagnosis groups, certain HPV genotypes included in the test, such as 6, 11, 16, 26, 35, 51, 58, 68, 73, and 89, which indicates possible variations at the species level.
conclusionsThere are HPV genotypes in Mexican population that cannot be detected by LA, which is, at present, the most complete commercial genotyping test. More studies are necessary to determine the impact of HPV-44, 74, 102 and 114 on the risk of developing CC. A greater number of samples must be analyzed by NGS for the most accurate determination of Mexican HPV variants.
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