ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016
Hydrogel biophysical properties instruct coculture-mediated osteogenic potential.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- A Review Into the Insights of the Role of Endothelial Progenitor Cells on Bone Biology.Frontiers in cell and developmental biology · 2022Review
- Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates.Science advances · 2020Article
- Engineering microenvironments towards harnessing pro-angiogenic potential of mesenchymal stem cells.Materials science & engineering. C, Materials for biological applications · 2019Article
- Matrix stiffness modulates the differentiation of neural crest stem cells in vivo.Journal of cellular physiology · 2019Article
- Exogenous Lysyl Oxidase-Like 2 and Perfusion Culture Induce Collagen Crosslink Formation in Osteogenic Grafts.Biotechnology journal · 2019Article
- Cell-secreted extracellular matrix, independent of cell source, promotes the osteogenic differentiation of human stromal vascular fraction.Journal of materials chemistry. B · 2018Article
- Nondestructive assessment of collagen hydrogel cross-linking using time-resolved autofluorescence imaging.Journal of biomedical optics · 2018Article
- Engineering fibrin hydrogels to promote the wound healing potential of mesenchymal stem cell spheroids.Acta biomaterialia · 2017Article
- Bioreactor culture duration of engineered constructs influences bone formation by mesenchymal stem cells.Biomaterials · 2017Article
- Multifactorial Experimental Design to Optimize the Anti-Inflammatory and Proangiogenic Potential of Mesenchymal Stem Cell Spheroids.Stem cells (Dayton, Ohio) · 2017Article
- A review of fibrin and fibrin composites for bone tissue engineering.International journal of nanomedicine · 2017Review
- Double-layered cell transfer technology for bone regeneration.Scientific reports · 2016Article
- Mesenchymal Stem Cell Spheroids Retain Osteogenic Phenotype Through α2β1 Signaling.Stem cells translational medicine · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Cell-based approaches for bone formation require instructional cues from the surrounding environment. As an alternative to pharmacological strategies or transplanting single cell populations, one approach is to coimplant populations that can establish a new vasculature and differentiate to bone-forming osteoblasts. Mesenchymal stem/stromal cells (MSCs) possess osteogenic potential and produce numerous angiogenic growth factors. Endothelial colony-forming cells (ECFCs) are a subpopulation of endothelial progenitor cells capable of vasculogenesis in vivo and may provide endogenous cues to support MSC function. We investigated the contribution of the carrier biophysical properties to instruct entrapped human MSCs and ECFCs to simultaneously promote their osteogenic and proangiogenic potential. Compared with gels containing MSCs alone, fibrin gels engineered with increased compressive stiffness simultaneously increased the osteogenic and proangiogenic potential of entrapped cocultured cells. ECFCs produced bone morphogenetic protein-2 (BMP-2), a potent osteoinductive molecule, and increases in BMP-2 secretion correlated with gel stiffness. Coculture of MSCs with ECFCs transduced to knockdown BMP-2 production abrogated the osteogenic response to levels observed with MSCs alone. These results demonstrate that physical properties of engineered hydrogels modulate the function of cocultured cells in the absence of inductive cues, thus increasing the translational potential of coimplantation to speed bone formation and repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.