ArticlePlant physiology2015
RNA Silencing of Exocyst Genes in the Stigma Impairs the Acceptance of Compatible Pollen in Arabidopsis.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Assessing the relative contributions of different Arabidopsis thaliana stigma factors to pollen hydration.Plant reproduction · 2026Article
- From recognition to response: integrated signaling pathways determining pollen acceptance and rejection in Brassicaceae.The New phytologist · 2026Review
- Transcriptional signatures associated with female receptivity and longevity in genetically male-sterile wheat (Triticum aestivum L.).Scientific reports · 2026Article
- Characterization of long non-coding RNAs during compatible and incompatible pollination inFrontiers in plant science · 2026Article
- The Arabidopsis SNARE complex genes regulate the early stages of pollen-stigma interactions.Plant reproduction · 2024Article
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- The chromosome-level genome of the submerged plant Cryptocoryne crispatula provides insights into the terrestrial-freshwater transition in Araceae.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Self-incompatibility: a targeted, unexplored pre-fertilization barrier in flower crops of Asteraceae.Journal of plant research · 2023Review
- Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence.Nature communications · 2023Article
- Recurrent Plant-Specific Duplications of KNL2 and Its Conserved Function as a Kinetochore Assembly Factor.Molecular biology and evolution · 2022Article
- Autophagy is required for self-incompatible pollen rejection in two transgenic Arabidopsis thaliana accessions.Plant physiology · 2022Article
- Communication is key: extracellular vesicles as mediators of infection and defence during host-microbe interactions in animals and plants.FEMS microbiology reviews · 2022Review
- Links between Regulatory Systems of ROS and Carbohydrates in Reproductive Development.Plants (Basel, Switzerland) · 2021Review
- Exocyst subunit Exo70B2 is linked to immune signaling and autophagy.The Plant cell · 2021Article
- Identification of Finely Mapped Quantitative Trait Locus and Candidate Gene Mining for the Three-Seeded Pod Trait in Soybean.Frontiers in plant science · 2021Article
- The Exocyst Complex Subunit EXO70E1-V FromFrontiers in plant science · 2021Article
- Expression of Brassica napus GLO1 is sufficient to breakdown artificial self-incompatibility in Arabidopsis thaliana.Plant reproduction · 2020Article
- Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O-arabinosyltransferase mutants.The Plant journal : for cell and molecular biology · 2020Article
- COPI complex isoforms are required for the early acceptance of compatible pollen grains in Arabidopsis thaliana.Plant reproduction · 2020Article
- Pollen-stigma interactions in Brassicaceae: complex communication events regulating pollen hydration.Journal of experimental botany · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initial pollen-pistil interactions in the Brassicaceae are regulated by rapid communication between pollen grains and stigmatic papillae and are fundamentally important, as they are the first step toward successful fertilization. The goal of this study was to examine the requirement of exocyst subunits, which function in docking secretory vesicles to sites of polarized secretion, in the context of pollen-pistil interactions. One of the exocyst subunit genes, EXO70A1, was previously identified as an essential factor in the stigma for the acceptance of compatible pollen in Arabidopsis (Arabidopsis thaliana) and Brassica napus. We hypothesized that EXO70A1, along with other exocyst subunits, functions in the Brassicaceae dry stigma to deliver cargo-bearing secretory vesicles to the stigmatic papillar plasma membrane, under the pollen attachment site, for pollen hydration and pollen tube entry. Here, we investigated the functions of exocyst complex genes encoding the remaining seven subunits, SECRETORY3 (SEC3), SEC5, SEC6, SEC8, SEC10, SEC15, and EXO84, in Arabidopsis stigmas following compatible pollinations. Stigma-specific RNA-silencing constructs were used to suppress the expression of each exocyst subunit individually. The early postpollination stages of pollen grain adhesion, pollen hydration, pollen tube penetration, seed set, and overall fertility were analyzed in the transgenic lines to evaluate the requirement of each exocyst subunit. Our findings provide comprehensive evidence that all eight exocyst subunits are necessary in the stigma for the acceptance of compatible pollen. Thus, this work implicates a fully functional exocyst complex as a component of the compatible pollen response pathway to promote pollen acceptance.
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