ArticlePlant & cell physiology2015
Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway.
Article in Plant & cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed.
- Article
- SnRK2.4 and SnRK2.10 redundantly control developmental leaf senescence by sustaining ABA production and signaling.Plant physiology · 2026Article
- Transcriptomic and metabolomic response of the Xunhua pepper landrace to BBWV2 infection in Qinghai Province, China.BMC plant biology · 2026Article
- Enhancing drought resilience in crops: mechanistic approaches in the face of climate challenge.Plant molecular biology · 2025Review
- Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- LEA proteins and ABA signaling: reciprocal regulation in stress adaptation.Frontiers in plant science · 2025Review
- Overexpression of MEKK18 from Arabidopsis pumila in rice significantly enhances stress resistance at the early stage.PloS one · 2025Article
- The MKK3 module integrates nitrate and light signals to modulate secondary dormancy inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Arabidopsis HECT and RING-type E3 Ligases Promote MAPKKK18 Degradation to Regulate Abscisic Acid Signaling.Plant & cell physiology · 2024Article
- MKK3 Cascade Regulates Seed Dormancy Through a Negative Feedback Loop Modulating ABA Signal in Rice.Rice (New York, N.Y.) · 2024Article
- Article
- Comparative transcriptome analysis reveals candidate genes for cold stress response and early flowering in pineapple.Scientific reports · 2023Article
- A pervasive phosphorylation cascade modulation of plant transcription factors in response to abiotic stress.Planta · 2023Review
- Transcriptomic Profiling Highlights the ABA Response Role ofInternational journal of molecular sciences · 2023Article
- The abscisic acid-responsive element binding factors MAPKKK18 module regulates abscisic acid-induced leaf senescence in Arabidopsis.The Journal of biological chemistry · 2023Article
- Evolution of theInternational journal of molecular sciences · 2023Article
- Identification of candidate regulators of the response to early heat stress in climate-adapted wheat landracesFrontiers in plant science · 2023Article
- The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis.Frontiers in plant science · 2023Article
- Harnessing the role of mitogen-activated protein kinases against abiotic stresses in plants.Frontiers in plant science · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation and dephosphorylation events play an important role in the transmission of the ABA signal. Although SnRK2 [sucrose non-fermenting1-related kinase2] protein kinases and group A protein phosphatase type 2C (PP2C)-type phosphatases constitute the core ABA pathway, mitogen-activated protein kinase (MAPK) pathways are also involved in plant response to ABA. However, little is known about the interplay between MAPKs and PP2Cs or SnRK2 in the regulation of ABA pathways. In this study, an effort was made to elucidate the role of MAP kinase kinase kinase18 (MKKK18) in relation to ABA signaling and response. The MKKK18 knockout lines showed more vigorous root growth, decreased abaxial stomatal index and increased stomatal aperture under normal growth conditions, compared with the control wild-type Columbia line. In addition to transcriptional regulation of the MKKK18 promoter by ABA, we demonstrated using in vitro and in vivo kinase assays that the kinase activity of MKKK18 was regulated by ABA. Analysis of the cellular localization of MKKK18 showed that the active kinase was targeted specifically to the nucleus. Notably, we identified abscisic acid insensitive 1 (ABI1) PP2C as a MKKK18-interacting protein, and demonstrated that ABI1 inhibited its activity. Using a cell-free degradation assay, we also established that MKKK18 was unstable and was degraded by the proteasome pathway. The rate of MKKK18 degradation was delayed in the ABI1 knockout line. Overall, we provide evidence that ABI1 regulates the activity and promotes proteasomal degradation of MKKK18.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.