ArticleScientific reports2015
An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 113 citations in OpenAlex.
- Val109 and Ile170 contribute to channel function in Guillardia theta-derived anion channelrhodopsins.Scientific reports · 2026Article
- Theoretical analysis of low-power optogenetic suppression of action potentials in human ventricular cardiomyocytes expressed with potassium-selective channelrhodopsins.Scientific reports · 2026Article
- Glutamatergic projection neurons in the basal forebrain underlie learned olfactory associational valence assignments.Nature communications · 2026Article
- Implantable Light-Powered Human Designer Cells for Electrical Energy Generation.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Neural vs Neuromorphic Interfaces: Where Are We Standing?Chemical reviews · 2025Review
- Pore-Opening and Ion-Conduction Mechanism in Channelrhodopsins C1C2, ChR2, and iChloC by Computational Electrophysiology and Constant-pH Simulations.Journal of chemical information and modeling · 2025Article
- Distinct Patterns of PV and SST GABAergic Neuronal Activity in the Basal Forebrain during Olfactory-Guided Behavior in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Review
- Review
- Sodium-Selective Channelrhodopsins.Cells · 2024Review
- Tonically active GABAergic neurons in the dorsal periaqueductal gray control instinctive escape in mice.Current biology : CB · 2024Article
- Two Ascending Thermosensory Pathways from the Lateral Parabrachial Nucleus That Mediate Behavioral and Autonomous Thermoregulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Potassium-selective channelrhodopsins.Biophysics and physicobiology · 2023Article
- Tailoring baker's yeast Saccharomyces cerevisiae for functional testing of channelrhodopsin.PloS one · 2023Article
- Parabrachial-to-parasubthalamic nucleus pathway mediates fear-induced suppression of feeding in male mice.Nature communications · 2022Article
- ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.Nature communications · 2022Article
- Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury.Circulation · 2022Article
- Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing.Communications biology · 2022Article
- Review
- Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Channelrhodopsins are light-gated cation channels that have been widely used for optogenetic stimulation of electrically excitable cells. Replacement of a glutamic acid in the central gate with a positively charged amino acid residue reverses the ion selectivity and produces chloride-conducting ChRs (ChloCs). Expressed in neurons, published ChloCs produced a strong shunting effect but also a small, yet significant depolarization from the resting potential. Depending on the state of the neuron, the net result of illumination might therefore be inhibitory or excitatory with respect to action potential generation. Here we report two additional amino acid substitutions that significantly shift the reversal potential of improved ChloC (iChloC) to the reversal potential of endogenous GABAA receptors. As a result, light-evoked membrane depolarization was strongly reduced and spike initiation after current injection or synaptic stimulation was reliably inhibited in iChloC-transfected neurons in vitro. In the primary visual cortex of anesthetized mice, activation of iChloC suppressed spiking activity evoked by visual stimulation. Due to its high operational light sensitivity, iChloC makes it possible to inhibit neurons in a large volume of brain tissue from a small, point-like light source.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.