ArticleScientific reports2015
Enzyme-linked DNA dendrimer nanosensors for acetylcholine.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Molecular fMRI of neurochemical signaling.Journal of neuroscience methods · 2021Review
- Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dendrimers as Modulators of Brain Cells.Molecules (Basel, Switzerland) · 2020Review
- Recent Developments in Nanosensors for Imaging Applications in Biological Systems.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Precision engineering of targeted nanocarriers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Nanosensors for the Chemical Imaging of Acetylcholine Using Magnetic Resonance Imaging.ACS nano · 2018Article
- Optical Probes for Neurobiological Sensing and Imaging.Accounts of chemical research · 2018Review
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is currently difficult to measure small dynamics of molecules in the brain with high spatial and temporal resolution while connecting them to the bigger picture of brain function. A step towards understanding the underlying neural networks of the brain is the ability to sense discrete changes of acetylcholine within a synapse. Here we show an efficient method for generating acetylcholine-detecting nanosensors based on DNA dendrimer scaffolds that incorporate butyrylcholinesterase and fluorescein in a nanoscale arrangement. These nanosensors are selective for acetylcholine and reversibly respond to levels of acetylcholine in the neurophysiological range. This DNA dendrimer architecture has the potential to overcome current obstacles to sensing in the synaptic environment, including the nanoscale size constraints of the synapse and the ability to quantify the spatio-temporal fluctuations of neurotransmitter release. By combining the control of nanosensor architecture with the strategic placement of fluorescent reporters and enzymes, this novel nanosensor platform can facilitate the development of new selective imaging tools for neuroscience.
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Registered trials
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