ArticleScientific reports2015
Direct regulation of the natural competence regulator gene tfoX by cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Vibrios.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- A Spontaneous Mutation in tfoX Impairs Bioluminescence in Photobacterium kishitanii ANT-2200.Molecular microbiology · 2026Article
- Rapid Preparation of Electrocompetent Listeria monocytogenes and Enhancement of Transformation Efficiency with cAMP Supplementation.Journal of food protection · 2026Article
- TseCurrent research in microbial sciences · 2026Article
- Quorum sensing regulators AphA and HapR differentially regulate fluvibactin biosynthesis inFrontiers in microbiology · 2026Article
- Characterization of quorum regulatory small RNAs in an emerging pathogenEmerging microbes & infections · 2024Article
- A Single Base Change in theMicroorganisms · 2024Article
- The ArgR-Regulated ADI Pathway Facilitates the Survival ofInternational journal of molecular sciences · 2024Article
- DegS regulates the aerobic metabolism ofFrontiers in cellular and infection microbiology · 2024Article
- Diverse regulatory pathways modulate bet hedging of competence induction in epigenetically-differentiated phase variants of Streptococcus pneumoniae.Nucleic acids research · 2023Article
- The molecular mechanism for carbon catabolite repression of the chitin response in Vibrio cholerae.PLoS genetics · 2023Article
- DegS protease regulates the motility, chemotaxis, and colonization ofFrontiers in microbiology · 2023Article
- Serotype conversion geneFrontiers in microbiology · 2023Article
- DegS protease regulates antioxidant capacity and adaptability to oxidative stress environment inFrontiers in cellular and infection microbiology · 2023Article
- CqsA/LuxS-HapR Quorum sensing circuit modulates type VI secretion system VflT6SS2 inEmerging microbes & infections · 2021Article
- Natural Transformation in a Classical-Biotype Vibrio cholerae Strain.Applied and environmental microbiology · 2021Article
- Fur RepressesFrontiers in microbiology · 2020Article
- Direct Binding and Regulation by Fur and HapR of the Intermediate Regulator and Virulence Factor Genes Within the ToxR Virulence Regulon inFrontiers in microbiology · 2020Article
- The quorum sensing transcription factor AphA directly regulates natural competence in Vibrio cholerae.PLoS genetics · 2019Article
- cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization.mBio · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TfoX (Sxy) and CRP are two important competence activators. The link between tfoX and CRP has been shown in H. influenza but lacking evidence of direct interaction. Recently a Sxy-dependent CRP (CRP-S) site autoregulating Sxy was reported in E. coli. Here, we show that the cAMP-CRP complex transcriptionally regulates tfoX expression through multiple canonical CRP (CRP-N) sites in Vibrios. This conclusion is supported by an analysis of the tfoX mRNA levels and tfoX transcriptional reporter fusions. The reduced expression of tfoX(VC) was restored by trans-complementation of crp in ∆crp and by exogenous cAMP in ∆cya. A promoter deletion analysis and the site-directed mutagenesis of the putative CRP-N sites revealed the presence of two functional CRP-N sites. The direct binding of cAMP-CRP to the tfoX(VC)promoter was demonstrated by EMSA assays. Additionally, the transcriptional start site (TSS) of tfoX(VF) in V. fluvialis was determined, and -10/-35 regions were predicted. Further comparison of the tfoX promoter in Vibrios revealed the existence of similar -10 motifs and putative CRP-N sites, indicating the conserved mechanism of CRP regulation on tfoX. Our study demonstrates the direct binding of the cAMP-CRP complex to tfoX promoter, and broadens the understanding of the molecular mechanism regulating tfoX in Vibrios.
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