ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2016
Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Insights into the zebrafish left-right organizer's centrosomes and cilia via volume electron microscopy.Biology open · 2026Article
- Molecular and Functional Divergence of Zebrafish Sox Paralogs Controlling Endoderm Formation and Left-Right Patterning.Genome biology and evolution · 2025Article
- Article
- Emx2 is an essential regulator of ciliated cell development across embryonic tissues.iScience · 2024Article
- Natural reversal of cavefish heart asymmetry is controlled by Sonic Hedgehog effects on the left-right organizer.Development (Cambridge, England) · 2024Article
- Emerging principles of primary cilia dynamics in controlling tissue organization and function.The EMBO journal · 2023Review
- Fluid extraction from the left-right organizer uncovers mechanical properties needed for symmetry breaking.eLife · 2023Article
- Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development.PLoS genetics · 2023Article
- LOF variants identifying candidate genes of laterality defects patients with congenital heart disease.PLoS genetics · 2022Article
- CARMIL3 is important for cell migration and morphogenesis during early development in zebrafish.Developmental biology · 2022Article
- Understanding laterality disorders and the left-right organizer: Insights from zebrafish.Frontiers in cell and developmental biology · 2022Review
- 3D viscoelastic drag forces contribute to cell shape changes during organogenesis in the zebrafish embryo.Cells & development · 2021Article
- Zebrafish Model as a Screen to Prevent Cyst Inflation in Autosomal Dominant Polycystic Kidney Disease.International journal of molecular sciences · 2021Article
- Simulations of particle tracking in the oligociliated mouse node and implications for left-right symmetry-breaking mechanics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Article
- Functional analysis of new human Bardet-Biedl syndrome loci specific variants in the zebrafish model.Scientific reports · 2019Article
- Aplnra/b Sequentially Regulate Organ Left-Right Patterning via Distinct Mechanisms.International journal of biological sciences · 2019Article
- Vertebrate myosin 1d regulates left-right organizer morphogenesis and laterality.Nature communications · 2018Article
- Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry.Nature communications · 2018Article
- Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle.eLife · 2018Article
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5 authors.
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Abstract
backgroundMotile cilia in the "organ of asymmetry" create directional fluid flows that are vital for left-right (LR) asymmetric patterning of vertebrate embryos. Organ function often depends on tightly regulated organ size control, but the role of organ of asymmetry size in LR patterning has remained unknown. Observations of the organ of asymmetry in the zebrafish, called Kupffer's vesicle (KV), have suggested significant variations in KV size in wild-type embryos, raising questions about the impact of KV organ size on LR patterning.
resultsTo understand the relationship between organ of asymmetry size and its function, we characterized variations in KV at several developmental stages and in several different zebrafish strains. We found that the number of KV cilia and the size of the KV lumen were highly variable, whereas the length of KV cilia showed less variation. These variabilities were similar among different genetic backgrounds. By specifically modulating KV size and analyzing individual embryos, we identified a size threshold that is necessary for KV function.
conclusionsTogether these results indicate the KV organ of asymmetry size is not tightly controlled during development, but rather must only exceed a threshold to direct robust LR patterning of the zebrafish embryo.
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