ArticleFrontiers in plant science2015
An improved high-throughput screening assay for tunicamycin sensitivity in Arabidopsis seedlings.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Network Biology Analyses and Dynamic Modeling of Gene Regulatory Networks under Drought Stress Reveal Major Transcriptional Regulators inInternational journal of molecular sciences · 2023Article
- The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity.Plant signaling & behavior · 2022Article
- Endoplasmic Reticulum Stress and Unfolded Protein Response Signaling in Plants.International journal of molecular sciences · 2022Review
- Advanced genomics identifies growth effectors for proteotoxic ER stress recovery in Arabidopsis thaliana.Communications biology · 2022Article
- Phytochrome B Positively Regulates Red Light-Mediated ER Stress Response inFrontiers in plant science · 2022Article
- CRISPR/Cas9 and Transgene Verification of Gene Involvement in Unfolded Protein Response and Recombinant Protein Production in Barley Grain.Frontiers in plant science · 2021Article
- A QuantitativeFrontiers in plant science · 2021Article
- Probing natural variation of IRE1 expression and endoplasmic reticulum stress responses in Arabidopsis accessions.Scientific reports · 2020Article
- A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants.Plant methods · 2020Article
- AtIRE1C, an unconventional isoform of the UPR master regulator AtIRE1, is functionally associated with AtIRE1B in Arabidopsis gametogenesis.Plant direct · 2019Article
- Recovery from temporary endoplasmic reticulum stress in plants relies on the tissue-specific and largely independent roles of bZIP28 and bZIP60, as well as an antagonizing function of BAX-Inhibitor 1 upon the pro-adaptive signaling mediated by bZIP28.The Plant journal : for cell and molecular biology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tunicamycin (Tm) sensitivity assays are a useful method for studies of endoplasmic reticulum stress and the unfolded protein response in eukaryotic cells. While Tm sensitivity and Tm recovery assays have been previously described, these existing methods are time-consuming, labor intensive, and subjected to mechanical wounding. This study shows an improved method of testing Tm sensitivity in Arabidopsis using liquid Murashige and Skoog medium versus the traditional solid agar plates. Liquid medium bypasses the physical manipulation of seedlings, thereby eliminating the risk of potential mechanical damage and additional unwanted stress to seedlings. Seedlings were subjected to comparative treatments with various concentrations of Tm on both solid and liquid media and allowed to recover. Determination of fresh weight, chlorophyll contents analysis and qRT-PCR results confirm the efficacy of using liquid medium to perform quantitative Tm stress assays.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.