ArticleFrontiers in microbiology2015
Control regions for chromosome replication are conserved with respect to sequence and location among Escherichia coli strains.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Dispensability of extrinsic DnaA regulators inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The replication enhancer crtS depends on transcription factor Lrp for modulating binding of initiator RctB to ori2 of Vibrio cholerae.Nucleic acids research · 2024Article
- Read-through transcription of tRNA underlies the cell cycle-dependent dissociation of IHF from the DnaA-inactivating sequenceFrontiers in microbiology · 2024Article
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- Regulatory elements coordinating initiation of chromosome replication to theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Robust replication initiation from coupled homeostatic mechanisms.Nature communications · 2022Article
- New Insights into the Antimicrobial Action of Cinnamaldehyde towardsBiomolecules · 2021Article
- Iron chelation increases the tolerance of Escherichia coli to hyper-replication stress.Scientific reports · 2018Article
- Impact of Chromosomal Architecture on the Function and Evolution of Bacterial Genomes.Frontiers in microbiology · 2018Review
- Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach.Journal of visualized experiments : JoVE · 2017Article
- Features of genomic organization in a nucleotide-resolution molecular model of the Escherichia coli chromosome.Nucleic acids research · 2017Article
- Random versus Cell Cycle-Regulated Replication Initiation in Bacteria: Insights from Studying Vibrio cholerae Chromosome 2.Microbiology and molecular biology reviews : MMBR · 2017Review
- The DnaA Cycle inFrontiers in microbiology · 2017Review
- DNA Replication Control Is Linked to Genomic Positioning of Control Regions in Escherichia coli.PLoS genetics · 2016Article
- Multiple DNA Binding Proteins Contribute to Timing of Chromosome Replication in E. coli.Frontiers in molecular biosciences · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli, chromosome replication is initiated from oriC by the DnaA initiator protein associated with ATP. Three non-coding regions contribute to the activity of DnaA. The datA locus is instrumental in conversion of DnaA(ATP) to DnaA(ADP) (datA dependent DnaA(ATP) hydrolysis) whereas DnaA rejuvenation sequences 1 and 2 (DARS1 and DARS2) reactivate DnaA(ADP) to DnaA(ATP). The structural organization of oriC, datA, DARS1, and DARS2 were found conserved among 59 fully sequenced E. coli genomes, with differences primarily in the non-functional spacer regions between key protein binding sites. The relative distances from oriC to datA, DARS1, and DARS2, respectively, was also conserved despite of large variations in genome size, suggesting that the gene dosage of either region is important for bacterial growth. Yet all three regions could be deleted alone or in combination without loss of viability. Competition experiments during balanced growth in rich medium and during mouse colonization indicated roles of datA, DARS1, and DARS2 for bacterial fitness although the relative contribution of each region differed between growth conditions. We suggest that this fitness advantage has contributed to conservation of both sequence and chromosomal location for datA, DARS1, and DARS2.
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