ArticleFrontiers in microbiology2015
Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Exploring the biochemical and biological functions of copper radical oxidases in the vascular wilt phytopathogenApplied and environmental microbiology · 2026Article
- Molecular Insights into the Activation of a Fungal Copper Radical Oxidase by Peroxidases.Chem & bio engineering · 2026Article
- A Molecular Mechanism for the Reduction of the Cu Site in Lytic Polysaccharide Monooxygenases by Phenol Reductants.JACS Au · 2025Article
- Exploring the synergy between fungal CE15 glucuronoyl esterases and xylanases for lignocellulose saccharification.Biotechnology for biofuels and bioproducts · 2025Article
- Functional Carbohydrate-Active Enzymes Acquired by Horizontal Gene Transfer from Plants in the Whitefly Bemisia tabaci.Genome biology and evolution · 2025Article
- Unlocking soybean meal pectin recalcitrance using a multi-enzyme cocktail approach.Scientific reports · 2025Article
- Design of Plastic Binding Lytic Polysaccharide Monooxygenases via Modular Engineering.Chem & bio engineering · 2024Article
- Phylogenomics reveals the evolutionary origins of lichenization in chlorophyte algae.Nature communications · 2024Article
- The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- TheApplied and environmental microbiology · 2023Article
- Insights into peculiar fungal LPMO family members holding a short C-terminal sequence reminiscent of phosphate binding motifs.Scientific reports · 2023Article
- Enzymatically prepared alginate oligosaccharides improve broiler chicken growth performance by modulating the gut microbiota and growth hormone signals.Journal of animal science and biotechnology · 2023Article
- A fungal lytic polysaccharide monooxygenase is required for cell wall integrity, thermotolerance, and virulence of the fungal human pathogen Cryptococcus neoformans.PLoS pathogens · 2023Article
- Engineering Flocculation for Improved Tolerance and Production of d-Lactic Acid inJournal of fungi (Basel, Switzerland) · 2023Article
- Tandem metalloenzymes gate plant cell entry by pathogenic fungi.Science advances · 2022Article
- The Maize Pathogen Ustilago maydis Secretes Glycoside Hydrolases and Carbohydrate Oxidases Directed toward Components of the Fungal Cell Wall.Applied and environmental microbiology · 2022Article
- A survey of substrate specificity among Auxiliary Activity Family 5 copper radical oxidases.Cellular and molecular life sciences : CMLS · 2021Article
- Identification of Copper-Containing Oxidoreductases in the Secretomes of ThreeApplied and environmental microbiology · 2021Article
- A Putative Lignin Copper Oxidase fromJournal of fungi (Basel, Switzerland) · 2021Article
- Screening New Xylanase Biocatalysts from the Mangrove Soil Diversity.Microorganisms · 2021Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi are the predominant source of lignocellulolytic enzymes used in industry for the transformation of plant biomass into high-value molecules and biofuels. The rapidity with which new fungal genomic and post-genomic data are being produced is vastly outpacing functional studies. This underscores the critical need for developing platforms dedicated to the recombinant expression of enzymes lacking confident functional annotation, a prerequisite to their functional and structural study. In the last decade, the yeast Pichia pastoris has become increasingly popular as a host for the production of fungal biomass-degrading enzymes, and particularly carbohydrate-active enzymes (CAZymes). This study aimed at setting-up a platform to easily and quickly screen the extracellular expression of biomass-degrading enzymes in P. pastoris. We first used three fungal glycoside hydrolases (GHs) that we previously expressed using the protocol devised by Invitrogen to try different modifications of the original protocol. Considering the gain in time and convenience provided by the new protocol, we used it as basis to set-up the facility and produce a suite of fungal CAZymes (GHs, carbohydrate esterases and auxiliary activity enzyme families) out of which more than 70% were successfully expressed. The platform tasks range from gene cloning to automated protein purifications and activity tests, and is open to the CAZyme users' community.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.