ArticleFrontiers in microbiology2015
Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Integrating machine learning and artificial intelligence in the management of Acinetobacter infections: a narrative review.Infection · 2026Review
- Current Methods for Reliable Identification of Species in theMicroorganisms · 2025Review
- Identification of blaBMC research notes · 2024Article
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- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Combination of RCA and DNAzyme for Dual-Signal Isothermal Amplification of Exosome RNA.Molecules (Basel, Switzerland) · 2023Article
- Assessment of Effective Antimicrobial Regimens and Mortality-Related Risk Factors for Bloodstream Infections Caused by Carbapenem-ResistantInfection and drug resistance · 2023Article
- Carbapenem-resistantFrontiers in microbiology · 2022Review
- Recombinase-aided amplification-lateral flow dipstick assay-a specific and sensitive method for visual detection of avian infectious laryngotracheitis virus.Poultry science · 2021Article
- Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members ofFrontiers in molecular biosciences · 2021Article
- Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.BMC microbiology · 2020Article
- Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan.Annals of clinical microbiology and antimicrobials · 2020Article
- Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification inBurns & trauma · 2020Article
- Article
- Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaScientific reports · 2019Article
- Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients.BioMed research international · 2019Article
- First investigation of the presence ofInfection and drug resistance · 2019Article
- Epidemiological evaluation of an Acinetobacter baumannii outbreak observed at an intensive care unit.Saudi medical journal · 2018Article
- Acinetobacter baumannii bacteraemia in patients with haematological malignancy: a multicentre retrospective study from the Infection Working Party of Jiangsu Society of Hematology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Spread of carbapenem-resistant international clones of Acinetobacter baumannii in Turkey and Azerbaijan: a collaborative study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Observational
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12 authors.
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Abstract
Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of A. baumannii. Group 2 carbapenems have been considered the agents of choice for the treatment of multiple drug-resistant A. baumannii. But the prevalence of carbapenem-resistant A. baumannii (CRAB) has been steadily increasing in recent years. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of A. baumannii in clinical samples by using high-specificity primers of the bla OXA-51 gene. Then we investigated the OXA-carbapenemases molecular epidemiology of A. baumannii isolates in two comprehensive hospitals in Beijing. The results showed that the LAMP assay could detect target DNA within 60 min at 65°C. The detection limit was 50 pg/μl, which was about 10-fold greater than that of PCR. Furthermore, this method could distinguish A. baumannii from the homologous A. nosocomialis and A. pittii. A total of 228 positive isolates were identified by this LAMP-based method for A. baumannii from 335 intensive care unit patients with clinically suspected multi-resistant infections in two hospitals in Beijing. The rates of CRAB are on the rise and are slowly becoming a routine phenotype for A. baumannii. Among the CRABs, 92.3% harbored both the bla OXA-23 and bla OXA-51 genes. Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C. In conclusion, the LAMP method developed for detecting A. baumannii was faster and simpler than conventional PCR and has great potential for both point-of-care testing and basic research. We further demonstrated a high distribution of class D carbapenemase-encoding genes, mainly OXA-23, which presents an emerging threat in hospitals in China.
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