ArticleFrontiers in microbiology2015
Genetic determinants of heat resistance in Escherichia coli.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 145 citations in OpenAlex.
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- Evolution of pathogenic Escherichia coli harboring the transmissible locus of stress tolerance: from food sources to clinical environments.Scientific reports · 2025Article
- Methods for Screening and Isolating Extremely Heat-ResistantLife (Basel, Switzerland) · 2024Article
- Article
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- Characteristics of Shiga Toxin-ProducingToxins · 2023Article
- Socializing at the Air-Liquid Interface: a Functional Genomic Analysis on Biofilm-Related Genes during Pellicle Formation by Escherichia coli and Its Interaction with Aeromonas australiensis.Applied and environmental microbiology · 2023Article
- Heat-resistant and biofilm-forming Escherichia coli in pasteurized milk from Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- The Heat Is On: Modeling the Persistence of ESBL-ProducingFoods (Basel, Switzerland) · 2022Article
- A Comprehensive Review of Variability in the Thermal Resistance (D-Values) of Food-Borne Pathogens-A Challenge for Thermal Validation Trials.Foods (Basel, Switzerland) · 2022Review
- Cytoplasmic molecular chaperones in Pseudomonas species.Journal of microbiology (Seoul, Korea) · 2022Review
- Whole-Genome Sequencing Analyses of Heat-Resistant Escherichia coli Isolated from Brazilian Beef.Microbiology resource announcements · 2022Article
- Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-ProducingMicroorganisms · 2022Article
- Genetic Characteristics of the Transmissible Locus of Stress Tolerance (tLST) and tLST Harboring Escherichia coli as Revealed by Large-Scale Genomic Analysis.Applied and environmental microbiology · 2022Article
- Comparative genomic and phenotypic analyses of the virulence potential in Shiga toxin-producingFrontiers in cellular and infection microbiology · 2022Article
- NaturalizedFrontiers in microbiology · 2022Review
- Biofilm-Forming Capacity of Escherichia coli Isolated from Cattle and Beef Packing Plants: Relation to Virulence Attributes, Stage of Processing, Antimicrobial Interventions, and Heat Tolerance.Applied and environmental microbiology · 2021Article
- Genetic Determinants of Stress Resistance in Desiccated Salmonella enterica.Applied and environmental microbiology · 2021Article
- Genomic and Phenotypic Analysis of Heat and Sanitizer Resistance in Escherichia coli from Beef in Relation to the Locus of Heat Resistance.Applied and environmental microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli AW1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. To identify the genetic determinants of heat resistance, 29 strains of E. coli that differed in their of heat resistance were analyzed by comparative genomics. Strains were classified as highly heat resistant strains, exhibiting a D60-value of more than 6 min; moderately heat resistant strains, exhibiting a D60-value of more than 1 min; or as heat sensitive. A ~14 kb genomic island containing 16 predicted open reading frames encoding putative heat shock proteins and proteases was identified only in highly heat resistant strains. The genomic island was termed the locus of heat resistance (LHR). This putative operon is flanked by mobile elements and possesses >99% sequence identity to genomic islands contributing to heat resistance in Cronobacter sakazakii and Klebsiella pneumoniae. An additional 41 LHR sequences with >87% sequence identity were identified in 11 different species of β- and γ-proteobacteria. Cloning of the full length LHR conferred high heat resistance to the heat sensitive E. coli AW1.7ΔpHR1 and DH5α. The presence of the LHR correlates perfectly to heat resistance in several species of Enterobacteriaceae and occurs at a frequency of 2% of all E. coli genomes, including pathogenic strains. This study suggests the LHR has been laterally exchanged among the β- and γ-proteobacteria and is a reliable indicator of high heat resistance in E. coli.
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