ArticleFrontiers in microbiology2015
Regulation of arsenite oxidation by the phosphate two-component system PhoBR in Halomonas sp. HAL1.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 40 citations in OpenAlex.
- Ferruginous hemeprotein HhuH facilitates the cadmium adsorption and chromium reduction inApplied and environmental microbiology · 2025Article
- Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli.International journal of molecular sciences · 2024Article
- Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacteriumFrontiers in microbiology · 2022Article
- Complete genome sequencing and comparative genomic analyses of Bacillus sp. S3, a novel hyper Sb(III)-oxidizing bacterium.BMC microbiology · 2020Article
- Microbial Oxidation of Arsenite: Regulation, Chemotaxis, Phosphate Metabolism and Energy Generation.Frontiers in microbiology · 2020Review
- Efflux proteins MacAB confer resistance to arsenite and penicillin/macrolide-type antibiotics in Agrobacterium tumefaciens 5A.World journal of microbiology & biotechnology · 2019Article
- Arsenite Oxidation by a Newly Isolated Betaproteobacterium PossessingFrontiers in microbiology · 2019Article
- Efflux Transporter ArsK Is Responsible for Bacterial Resistance to Arsenite, Antimonite, Trivalent Roxarsone, and Methylarsenite.Applied and environmental microbiology · 2018Article
- The CytochromeFrontiers in microbiology · 2018Article
- Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4.Scientific reports · 2017Article
- Abiotic and biotic factors responsible for antimonite oxidation in Agrobacterium tumefaciens GW4.Scientific reports · 2017Article
- An Oxidoreductase AioE is Responsible for Bacterial Arsenite Oxidation and Resistance.Scientific reports · 2017Article
- Regulation of Class A β-Lactamase CzoA by CzoR and IscR inFrontiers in microbiology · 2017Article
- Disrupting ROS-protection mechanism allows hydrogen peroxide to accumulate and oxidize Sb(III) to Sb(V) in Pseudomonas stutzeri TS44.BMC microbiology · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, the expression of arsenite [As(III)] oxidase genes aioBA was reported to be regulated by a three-component regulatory system, AioXSR, in a number of As(III)-oxidizing bacterial strains. However, the regulation mechanism is still unknown when aioXSR genes are absent in some As(III)-oxidizing bacterial genomes, such as in Halomonas sp. HAL1. In this study, transposon mutagenesis and gene knock-out mutation were performed, and two mutants, HAL1-phoR 931 and HAL1-▵phoB, were obtained in strain HAL1. The phoR and phoB constitute a two-component system which is responsible for phosphate (Pi) acquisition and assimilation. Both of the mutants showed negative As(III)-oxidation phenotypes in low Pi condition (0.1 mM) but not under normal Pi condition (1 mM). The phoBR complementation strain HAL1-▵phoB-C reversed the mutants' null phenotypes back to wild type status. Meanwhile, lacZ reporter fusions using pCM-lacZ showed that the expression of phoBR and aioBA were both induced by As(III) but were not induced in HAL1-phoR 931 and HAL1-▵phoB. Using 15 consensus Pho box sequences, a putative Pho box was found in the aioBA regulation region. PhoB was able to bind to the putative Pho box in vivo (bacterial one-hybrid detection) and in vitro (electrophoretic mobility gel shift assay), and an 18-bp binding sequence containing nine conserved bases were determined. This study provided the evidence that PhoBR regulates the expression of aioBA in Halomonas sp. HAL1 under low Pi condition. The new regulation model further implies the close metabolic connection between As and Pi.
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