ArticleFrontiers in microbiology2015
Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation-inducible promoter from Chlamydomonas reinhardtii.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 78 citations in OpenAlex.
- Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in Nannochloropsis oceanica (Eustigmatophyceae).Journal of phycology · 2026Article
- Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system.Scientific reports · 2025Article
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- Lipid Droplets in EndosymbioticPlants (Basel, Switzerland) · 2024Review
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- Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica.Microbial cell factories · 2023Article
- Nutritional Composition and Untargeted Metabolomics Reveal the Potential ofAnimals : an open access journal from MDPI · 2022Article
- Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga.Scientific reports · 2022Article
- Microalgae Biomass as a New Potential Source of Sustainable Green Lubricants.Molecules (Basel, Switzerland) · 2022Review
- Comparative Proteomics Reveals Evidence of Enhanced EPA Trafficking in a Mutant Strain ofFrontiers in bioengineering and biotechnology · 2022Article
- Nutritional conditions of the novel freshwater Coccomyxa AP01 for versatile fatty acids composition.Journal of applied microbiology · 2022Article
- Characterization of the Bubblegum acyl-CoA synthetase of Microchloropsis gaditana.Plant physiology · 2021Article
- Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Lipid Droplets in Unicellular Photosynthetic Stramenopiles.Frontiers in plant science · 2021Review
- The MicroalgaPlant physiology · 2020Article
- Molecular profiling of an oleaginous trebouxiophycean algaBiotechnology for biofuels · 2019Article
- Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.Plant cell reports · 2018Review
- Betaine Lipid Is Crucial for Adapting to Low Temperature and Phosphate Deficiency inPlant physiology · 2018Article
- Review
- Engineering the Chloroplast Genome of Oleaginous Marine MicroalgaFrontiers in plant science · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae accumulate triacylglycerols (TAGs) under conditions of nutrient stress. Phosphorus (P) starvation induces the accumulation of TAGs, and the cells under P starvation maintain growth through photosynthesis. We recently reported that P starvation-dependent overexpression of type-2 diacylglycerol acyl-CoA acyltransferase (CrDGTT4) from Chlamydomonas reinhardtii using a sulfoquinovosyldiacylglycerol synthase 2 (SQD2) promoter, which has increased activity during P starvation, enhances TAG accumulation in C. reinhardtii cells. As a result, the content of C18:1 fatty acid, a preferred substrate of CrDGTT4, is increased in TAGs. Here we isolated genes encoding SQD2 from strain NIES-2145 of the eustigmatophyte Nannochloropsis and showed that their expression, like that in C. reinhardtii, was up-regulated during P starvation. To enhance oil accumulation under P starvation, we transformed pCrSQD2-CrDGTT4 into Nannochloropsis strain NIES-2145. The transformants had a fatty acid composition that was more similar to that of C. reinhardtii, which resulted in enhanced TAG accumulation and higher 18:1(9) content. The results indicated that the P starvation-inducible promoter of C. reinhardtii was able to drive expression of the CrDGTT4 gene in Nannochloropsis strain NIES-2145 under P starvation. We conclude that the heterologous CrSQD2 promoter is effective in manipulating TAG synthesis in Nannochloropsis during P starvation.
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