ArticleFrontiers in cellular neuroscience2015
Tracking the activity-dependent diffusion of synaptic proteins using restricted photoconversion of Dendra2.
Article in Frontiers in cellular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Long-term tracing of individual human neural cells using multiphoton microscopy and photoconvertible polymer capsules.Journal of the Royal Society, Interface · 2024Article
- Temporally resolved SMLM (with large PAR shift) enabled visualization of dynamic HA cluster formation and migration in a live cell.Scientific reports · 2023Article
- Bidirectional regulation of synaptic SUMOylation by Group 1 metabotropic glutamate receptors.Cellular and molecular life sciences : CMLS · 2022Article
- The synaptic balance between sumoylation and desumoylation is maintained by the activation of metabotropic mGlu5 receptors.Cellular and molecular life sciences : CMLS · 2019Article
- Sumoylation regulates FMRP-mediated dendritic spine elimination and maturation.Nature communications · 2018Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
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Abstract
Spines are small protrusions on dendritic membranes receiving inputs from axonal termini. They consist in a head connected to the dendritic shaft by a narrow neck and contain multiple synaptic proteins that interact in a coordinated manner to allow for synaptic communication. This process involves many proteins that are moving in and out spines. However, comparing this synaptodendritic movement in basal and stimulated conditions is very challenging. Here we describe an elegant method to measure the activity-dependent diffusion of synaptic proteins using Dendra2 photoconversion. We provide a successful method to obtain Dendra2-photoconverted images and a step-by-step procedure to analyze the data. This live-imaging approach may also apply to investigate the diffusion of proteins across other subcellular compartments or organelles including but not restricted to, nucleus, nucleolus, ER, or vesicular structures. Once the imaging system is set up, data can be acquired in 1-30 min and analyzed in approximately 1-4 h.
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