ArticleEndocrinology2015
A High-Calcium and Phosphate Rescue Diet and VDR-Expressing Transgenes Normalize Serum Vitamin D Metabolite Profiles and Renal Cyp27b1 and Cyp24a1 Expression in VDR Null Mice.
Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Inhibit progression of coronary artery calcification with vitamin K in hemodialysis patients (the iPACK-HD study): a randomized, placebo-controlled multi-center, pilot trial.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2023Trial
- Myeloid cell expression of the VDR and parathyroid hormone control the host response to influenza infection in mice.Frontiers in immunology · 2026Article
- Vitamin D as an Immune Modulator in Systemic Lupus Erythematosus: A Narrative Review.Life (Basel, Switzerland) · 2025Review
- Kidney deletions of Cyp27b1 fail to reduce serum 1,25(OH)The Journal of steroid biochemistry and molecular biology · 2025Article
- Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs.Metabolites · 2022Article
- The metabolism of 1,25(OH)Scientific reports · 2022Article
- Prevention of Hypomineralization In Auditory Ossicles of Vitamin D Receptor (Vdr) Deficient Mice.Frontiers in endocrinology · 2022Article
- Renal functional, transcriptome, and methylome adaptations in pregnant Sprague Dawley and Brown Norway rats.PloS one · 2022Article
- Role of Vitamin D in Cognitive Dysfunction: New Molecular Concepts and Discrepancies between Animal and Human Findings.Nutrients · 2021Review
- Mechanical stress regulates bone regulatory gene expression independent of estrogen and vitamin D deficiency in rats.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- The Vitamin D Receptor in Osteoblast-Lineage Cells Is Essential for the Proresorptive Activity of 1α,25(OH)2D3 In Vivo.Endocrinology · 2020Article
- Review
- PTH suppression by calcitriol does not predict off-target actions in experimental CKD.Pharmacology research & perspectives · 2020Article
- Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice.Scientific reports · 2019Article
- A chromatin-based mechanism controls differential regulation of the cytochrome P450 geneThe Journal of biological chemistry · 2019Article
- Targeted genomic deletions identify diverse enhancer functions and generate a kidney-specific, endocrine-deficientThe Journal of biological chemistry · 2019Article
- Vitamin D Is Required for ILC3 Derived IL-22 and Protection FromFrontiers in immunology · 2019Article
- Hereditary Hypercalcemia Caused by a Homozygous Pathogenic Variant in theCase reports in endocrinology · 2019Article
- Role of phosphate sensing in bone and mineral metabolism.Nature reviews. Endocrinology · 2018Review
- Time Course of Vitamin D Depletion and Repletion in Reproductive-age Female C57BL/6 Mice.Comparative medicine · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Vitamin D receptor (VDR)-mediated 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-dependent gene expression is compromised in the VDR null mouse. The biological consequences include: hypocalcemia, hypophosphatemia, elevated parathyroid hormone (PTH) and 1,25(OH)2D3, and consequential skeletal abnormalities. CYP24A1 is a cytochrome P450 enzyme that is involved in the side chain oxidation and destruction of both 1,25(OH)2D3 and 25-hydroxyvitamin D3 (25-OH-D3). In the current studies, we used liquid chromatography-tandem mass spectrometry technology to compare the metabolic profiles of VDR null mice fed either a normal or a calcium and phosphate-enriched rescue diet and to assess the consequence of transgenic expression of either mouse or human VDR genes in the same background. Serum 1,25(OH)2D3 levels in VDR null mice on normal chow were highly elevated (>3000 pg/mL) coincident with undetectable levels of catabolites such as 24,25-(OH)2D3 and 25-OH-D3-26,23-lactone normally observed in wild-type mice. The rescue diet corrected serum Ca(++), PTH, and 1,25(OH)2D3 values and restored basal expression of Cyp24a1 as evidenced by both renal expression of Cyp24a1 and detection of 24,25-(OH)2D3 and the 25-OH-D3-26,23-lactone. Unexpectedly, this diet also resulted in supranormal levels of 3-epi-24,25-(OH)2D3 and 3-epi-25-OH-D3-26,23-lactone. The reappearance of serum 24,25-(OH)2D3 and renal Cyp24a1 expression after rescue suggests that basal levels of Cyp24a1 may be repressed by high PTH. Introduction of transgenes for either mouse or human VDR also normalized vitamin D metabolism in VDR null mice, whereas this metabolic pattern was unaffected by a transgene encoding a ligand binding-deficient mutant (L233S) human VDR. We conclude that liquid chromatography-tandem mass spectrometry-based metabolic profiling is an ideal analytical method to study mouse models with alterations in calcium/phosphate homeostasis.
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Registered trials
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