ArticleACS chemical biology2015
Bacterial Genome Mining of Enzymatic Tools for Alkyne Biosynthesis.
Article in ACS chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Terminal alkyne formation by a pyridoxal phosphate-dependent enzyme.Nature chemical biology · 2026Article
- Discovery, characterization and mechanism of a Microbacterium esterase for key d-biotin chiral intermediate synthesis.Bioresources and bioprocessing · 2024Article
- Biosynthesis of Isonitrile- and Alkyne-Containing Natural Products.Annual review of chemical and biomolecular engineering · 2022Review
- Integrated omics approach to unveil antifungal bacterial polyynes as acetyl-CoA acetyltransferase inhibitors.Communications biology · 2022Article
- Untargeted Identification of Alkyne-Containing Natural Products Using Ruthenium-Catalyzed Azide Alkyne Cycloaddition Reactions Coupled to LC-MS/MS.Journal of natural products · 2022Article
- Engineered biosynthesis of alkyne-tagged polyketides.Methods in enzymology · 2022Article
- Article
- Mining and unearthing hidden biosynthetic potential.Nature communications · 2021Review
- Biosynthesis of alkyne-containing natural products.RSC chemical biology · 2021Review
- Article
- Bioorganic chemistry of signaling molecules in microbial communication.Journal of pesticide science · 2019Article
- Volatile Compounds Produced by Cyanobacteria Isolated from Mangrove Environment.Current microbiology · 2019Article
- Article
- Probing the Acyl Carrier Protein-Enzyme Interactions within Terminal Alkyne Biosynthetic Machinery.AIChE journal. American Institute of Chemical Engineers · 2018Article
- Acetylenotrophy: a hidden but ubiquitous microbial metabolism?FEMS microbiology ecology · 2018Review
- Engineering modular polyketide synthases for production of biofuels and industrial chemicals.Current opinion in biotechnology · 2018Review
- Phylogeny-guided (meta)genome mining approach for the targeted discovery of new microbial natural products.Journal of industrial microbiology & biotechnology · 2017Review
- A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool.Chemical communications (Cambridge, England) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The alkyne is an important functionality widely used in material science, pharmaceutical science, and chemical biology, but the importance of this functionality is contrasted by the very limited number of enzymes known to be involved in alkyne biosynthesis. We recently reported the first known carrier protein-dependent pathway for terminal alkyne formation, and in silico analysis suggested that this mechanism could be widespread in bacteria. In this paper, we screened additional homologous gene cassettes presumed to be involved in alkyne biosynthesis using both in vitro biochemical study and an E. coli-polyketide synthase (PKS) reporting system for in vivo analysis. We discovered and characterized a new terminal alkyne biosynthetic pathway comprised of TtuA, -B, and -C from Teredinibacter turnerae T7901. While the acyl-CoA ligase homologue (TtuA) demonstrated promiscuity in the activation and loading of medium-chain fatty acids onto the carrier protein (TtuC), the desaturase homologue (TtuB) showed stringent substrate specificity toward C10 fatty acyl moieties. In addition, TtuB was demonstrated to be a bifunctional desaturase/acetylenase that efficiently catalyzed two sequential O2-dependent dehydrogenation reactions. A novel terminal-alkyne bearing polyketide was further produced upon coexpression of ttuABC and a PKS gene in E. coli. The discovery and characterization of TtuA, -B, and -C provides us with a new bifunctional desaturase/acetylenase for mechanistic and structural study and expands the scarce enzyme inventory for the biosynthesis of the alkyne functionality, which has important applications in synthetic and chemical biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.