ArticlePlant cell reports2016
Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Bioreactor Technology for Medicinal Plant In Vitro Cultures: Systems, Applications, and Future Perspectives.Biology · 2026Review
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- Compounds from medicinal plants produced in hairy root and transgenic hairy root cultures: a review.PeerJ · 2025Review
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Collection of Hairy Roots as a Basis for Fundamental and Applied Research.Molecules (Basel, Switzerland) · 2022Review
- Design of nutrient gas-phase bioreactors: a critical comprehensive review.Bioprocess and biosystems engineering · 2022Review
- Biotechnological Approaches for Production of Artemisinin, an Anti-Malarial Drug fromMolecules (Basel, Switzerland) · 2022Review
- Antimalarial Natural Products.Progress in the chemistry of organic natural products · 2022Article
- Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook.Applied microbiology and biotechnology · 2021Review
- Xanthones Production inPlants (Basel, Switzerland) · 2021Article
- Statistical optimization of bacoside A biosynthesis in plant cell suspension cultures using response surface methodology.3 Biotech · 2020Article
- Plant tissue culture as a perpetual source for production of industrially important bioactive compounds.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Biotic elicitors enhance diosgenin production inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata.Scientific reports · 2018Article
- Specialized Plant Metabolism Characteristics and Impact on Target Molecule Biotechnological Production.Molecular biotechnology · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageAlternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study featured investigation of growth and artemisinin production by A. annua hairy roots (induced by Agrobacterium rhizogenes-mediated genetic transformation of explants) in three bioreactor configurations-bubble column reactor, NMB and modified NMB particularly to establish their suitability for commercial production. It was observed that cultivation of hairy roots in a non-stirred bubble column reactor exhibited a biomass accumulation of 5.68 g/l only while batch cultivation in a custom-made NMB exhibited a higher biomass concentration of 8.52 g/l but relatively lower artemisinin accumulation of 0.22 mg/g was observed in this reactor. A mixture of submerged liquid-phase growth (for 5 days) followed by gas-phase cultivation in nutrient mist reactor operation strategy (for next 15 days) was adopted for hairy root cultivation in this investigation. Reasonably, high (23.02 g/l) final dry weight along with the artemisinin accumulation (1.12 mg/g, equivalent to 25.78 mg/l artemisinin) was obtained in this bioreactor, which is the highest reported artemisinin yield in the gas-phase NMB cultivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.