ArticlePlant cell reports2016
Arabidopsis NAC016 promotes chlorophyll breakdown by directly upregulating STAYGREEN1 transcription.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Development and research trends of stay-green biology in legumes: a bibliometric and visual analysis over three decades.Frontiers in genetics · 2026Pooled it
- Gene network analysis predicts the primary regulators of ABA-dependent transcriptional activation and repression in Populus roots.PLoS genetics · 2026Article
- Article
- The NAC transcription factor BrrNAC082 positively mediates leaf senescence in Brassica rapa.The Plant journal : for cell and molecular biology · 2026Article
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Identification of candidate genes associated with regreening stage in rapeseed (Brassica Rapa L.) by combined application of BSA-Seq and RNA-Seq.BMC plant biology · 2026Article
- The atypical Dof transcriptional factor OsDes1 contributes to stay-green, grain yield, and disease resistance in rice.Science advances · 2024Article
- The NAC factor LpNAL delays leaf senescence by repressing two chlorophyll catabolic genes in perennial ryegrass.Plant physiology · 2022Article
- MdNAC4 Interacts With MdAPRR2 to Regulate Nitrogen Deficiency-Induced Leaf Senescence in Apple (Frontiers in plant science · 2022Article
- Primary metabolic processes as drivers of leaf ageing.Cellular and molecular life sciences : CMLS · 2021Review
- Increased expression of ANAC017 primes for accelerated senescence.Plant physiology · 2021Article
- VviNAC33 promotes organ de-greening and represses vegetative growth during the vegetative-to-mature phase transition in grapevine.The New phytologist · 2021Article
- Identification of biological pathway and process regulators using sparse partial least squares and triple-gene mutual interaction.Scientific reports · 2021Article
- Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses.Scientific reports · 2021Article
- Article
- AtWAKL10, a Cell Wall Associated Receptor-Like Kinase, Negatively Regulates Leaf Senescence inInternational journal of molecular sciences · 2021Article
- The GIGANTEA-ENHANCED EM LEVEL Complex Enhances Drought Tolerance via Regulation of Abscisic Acid Synthesis.Plant physiology · 2020Article
- HEBE, a novel positive regulator of senescence in Solanum lycopersicum.Scientific reports · 2020Article
- Multilayered Regulation of Membrane-Bound ONAC054 Is Essential for Abscisic Acid-Induced Leaf Senescence in Rice.The Plant cell · 2020Article
- PheophorbidePlant physiology · 2020Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe Arabidopsis transcriptional factor NAC016 directly activates chlorophyll degradation during leaf senescence by binding to the promoter of SGR1 and upregulating its transcription. During leaf senescence or abiotic stress in Arabidopsis thaliana, STAYGREEN1 (SGR1) promotes chlorophyll (Chl) degradation, acting with Chl catabolic enzymes, but the mechanism regulating SGR1 transcription remains largely unknown. Here, we show that the Arabidopsis senescence-associated NAC transcription factor NAC016 directly activates SGR1 transcription. Under senescence-promoting conditions, the expression of SGR1 was downregulated in nac016-1 mutants and upregulated in NAC016-overexpressing (NAC016-OX) plants. By yeast one-hybrid and chromatin immunoprecipitation assays, we found that NAC016 directly binds to the SGR1 promoter, which contains the NAC016-specific binding motif (termed the NAC016BM). Furthermore, nac016-1 SGR1-OX plants showed an early leaf yellowing phenotype, similar to SGR1-OX plants, confirming that NAC016 directly activates SGR1 expression in the leaf senescence regulatory cascade. Although we found that NAC016 activates SGR1 expression in senescing leaves, this transcriptional regulation is considerably weaker in maturing seeds; the seeds of sgr1-1 mutants (also known as nonyellowing1-1, nye1-1) stayed green, while the seeds of nac016-1 mutants turned from green to yellow normally. We also found that the abscisic acid (ABA) signaling-related transcription factor genes ABI5 and EEL and the ABA biosynthesis gene AAO3, which activate SGR1 expression directly or indirectly, were significantly downregulated in nac016-1 mutants and upregulated in NAC016-OX plants. However, the NAC016BM does not exist in their promoter regions, indicating that NAC016 may indirectly activate these ABA signaling and biosynthesis genes, probably by directly activating transcriptional cascades regulated by the NAC transcription factor NAP. The NAC016-mediated regulatory cascades of SGR1 and other Chl degradation-related genes are discussed.
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