ArticlePloS one2015
High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- FirstFrontiers in plant science · 2024Article
- Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars.International journal of molecular sciences · 2022Article
- Article
- Genomics Associated Interventions for Heat Stress Tolerance in Cool Season Adapted Grain Legumes.International journal of molecular sciences · 2021Review
- Omics resources and omics-enabled approaches for achieving high productivity and improved quality in pea (Pisum sativum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing.Biological research · 2020Article
- SSR identification and marker development for sago palm based on NGS genome data.Breeding science · 2019Article
- Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Mycobiology · 2019Article
- Development of microsatellite markers for Myracrodruon urundeuva (F.F. & M.F. Allemão), a highly endangered species from tropical forest based on next-generation sequencing.Molecular biology reports · 2018Article
- Article
- Marker-trait association analysis of frost tolerance of 672 worldwide pea (Pisum sativum L.) collections.Scientific reports · 2017Article
- Molecular, Genetic and Agronomic Approaches to Utilizing Pulses as Cover Crops and Green Manure into Cropping Systems.International journal of molecular sciences · 2017Review
- Pea Marker Database (PMD) - A new online database combining known pea (Pisum sativum L.) gene-based markers.PloS one · 2017Article
- Development and Characterization of Novel Microsatellite Markers for the Peach Fruit Moth Carposina sasakii (Lepidoptera: Carposinidae) Using Next-Generation Sequencing.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population.
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