ArticlePloS one2015
Metabolomic Profiling of 13 Diatom Cultures and Their Adaptation to Nitrate-Limited Growth Conditions.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Statistics and Machine Learning in Mass Spectrometry-Based Metabolomics Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- 3D Natural Mesoporous Biosilica-Embedded Polysulfone Made Ultrafiltration Membranes for Application in Separation Technology.Polymers · 2022Article
- Host genotype structures the microbiome of a globally dispersed marine phytoplankton.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Effects of extraction parameters on lipid profiling of mosses using UPLC-ESI-QTOF-MS and multivariate data analysis.Metabolomics : Official journal of the Metabolomic Society · 2021Article
- Recent advances in the discovery of novel marine natural products and mycosporine-like amino acid UV-absorbing compounds.Applied microbiology and biotechnology · 2021Review
- Integrative omics identification, evolutionary and structural analysis of low affinity nitrate transporters in diatoms, diNPFs.Open biology · 2021Article
- The Contribution of Metabolomics to Systems Biology: Current Applications Bridging Genotype and Phenotype in Plant Science.Advances in experimental medicine and biology · 2021Article
- A Metabolomics Exploration of the Sexual Phase in the Marine DiatomMarine drugs · 2020Article
- Responses of Marine Diatom Skeletonema marinoi to Nutrient Deficiency: Programmed Cell Death.Applied and environmental microbiology · 2020Article
- Valuable Fatty Acids in Bryophytes-Production, Biosynthesis, Analysis and Applications.Plants (Basel, Switzerland) · 2019Review
- TiORSC advances · 2019Article
- Systematic Error Removal Using Random Forest for Normalizing Large-Scale Untargeted Lipidomics Data.Analytical chemistry · 2019Article
- AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms.Biotechnology for biofuels · 2018Article
- Article
- The effects of phosphorus limitation on carbon metabolism in diatoms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Molecular Identification and Comparative Evaluation of Tropical Marine Microalgae for Biodiesel Production.Marine biotechnology (New York, N.Y.) · 2017Article
- Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diatoms are very efficient in their use of available nutrients. Changes in nutrient availability influence the metabolism and the composition of the cell constituents. Since diatoms are valuable candidates to search for oil producing algae, measurements of diatom-produced compounds can be very useful for biotechnology. In order to explore the diversity of lipophilic compounds produced by diatoms, we describe the results from an analysis of 13 diatom strains. With the help of a lipidomics platform, which combines an UPLC separation with a high resolution/high mass accuracy mass spectrometer, we were able to measure and annotate 142 lipid species. Out of these, 32 were present in all 13 cultures. The annotated lipid features belong to six classes of glycerolipids. The data obtained from the measurements were used to create lipidomic profiles. The metabolomic overview of analysed cultures is amended by the measurement of 96 polar compounds. To further increase the lipid diversity and gain insight into metabolomic adaptation to nitrogen limitation, diatoms were cultured in media with high and low concentrations of nitrate. The growth in nitrogen-deplete or nitrogen-replete conditions affects metabolite accumulation but has no major influence on the species-specific metabolomic profile. Thus, the genetic component is stronger in determining metabolic patterns than nitrogen levels. Therefore, lipid profiling is powerful enough to be used as a molecular fingerprint for diatom cultures. Furthermore, an increase of triacylglycerol (TAG) accumulation was observed in low nitrogen samples, although this trend was not consistent across all 13 diatom strains. Overall, our results expand the current understanding of metabolomics diversity in diatoms and confirm their potential value for producing lipids for either bioenergy or as feed stock.
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