ArticlePloS one2015
Transcriptomic Analysis of Trout Gill Ionocytes in Fresh Water and Sea Water Using Laser Capture Microdissection Combined with Microarray Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Transcriptome-Based Analysis of the Effects of Salt Stress on the Embryos ofAnimals : an open access journal from MDPI · 2026Article
- Environmental and molecular control of tissue-specific ionocyte differentiation in zebrafish.Development (Cambridge, England) · 2024Article
- Endocrine control of gill ionocyte function in euryhaline fishes.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- Characterization of transcriptome changes in saline stress adaptation on Leuciscus merzbacheri using PacBio Iso-Seq and RNA-Seq.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Environmental and molecular control of tissue-specific ionocyte differentiation in zebrafish.bioRxiv : the preprint server for biology · 2024Article
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- Role of the kidneys in acid-base regulation and ammonia excretion in freshwater and seawater fish: implications for nephrocalcinosis.Frontiers in physiology · 2023Review
- NaJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022Article
- Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes.BMC genomics · 2022Article
- Mechanisms of NaFrontiers in physiology · 2022Review
- Sulfate homeostasis in Atlantic salmon is associated with differential regulation of salmonid-specific paralogs in gill and kidney.Physiological reports · 2021Article
- The time course of molecular acclimation to seawater in a euryhaline fish.Scientific reports · 2021Article
- Immunologic Profiling of the Atlantic Salmon Gill by Single Nuclei Transcriptomics.Frontiers in immunology · 2021Article
- Draft genome assembly of Tenualosa ilisha, Hilsa shad, provides resource for osmoregulation studies.Scientific reports · 2019Article
- Association Mapping Based on a Common-Garden Migration Experiment Reveals Candidate Genes for Migration Tendency in Brown Trout.G3 (Bethesda, Md.) · 2019Article
- Comparative transcriptome analysis reveals osmotic-regulated genes in the gill of Chinese mitten crab (Eriocheir sinensis).PloS one · 2019Article
- Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology.PloS one · 2017Article
- Flexible selection of diversified Na(+)/K(+)-ATPase α-subunit isoforms for osmoregulation in teleosts.Zoological letters · 2016Article
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5 authors.
Funding
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Abstract
Fish gills represent a complex organ composed of several cell types that perform multiple physiological functions. Among these cells, ionocytes are implicated in the maintenance of ion homeostasis. However, because the ionocyte represents only a small percent of whole gill tissue, its specific transcriptome can be overlooked among the numerous cell types included in the gill. The objective of this study is to better understand ionocyte functions by comparing the RNA expression of this cell type in freshwater and seawater acclimated rainbow trout. To realize this objective, ionocytes were captured from gill cryosections using laser capture microdissection after immunohistochemistry. Then, transcriptome analyses were performed on an Agilent trout oligonucleotide microarray. Gene expression analysis identified 108 unique annotated genes differentially expressed between freshwater and seawater ionocytes, with a fold change higher than 3. Most of these genes were up-regulated in freshwater cells. Interestingly, several genes implicated in ion transport, extracellular matrix and structural cellular proteins appeared up-regulated in freshwater ionocytes. Among them, several ion transporters, such as CIC2, SLC26A6, and NBC, were validated by qPCR and/or in situ hybridization. The latter technique allowed us to localize the transcripts of these ion transporters in only ionocytes and more particularly in the freshwater cells. Genes involved in metabolism and also several genes implicated in transcriptional regulation, cell signaling and the cell cycle were also enhanced in freshwater ionocytes. In conclusion, laser capture microdissection combined with microarray analysis allowed for the determination of the transcriptional signature of scarce cells in fish gills, such as ionocytes, and aided characterization of the transcriptome of these cells in freshwater and seawater acclimated trout.
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