ArticleArchives of oral biology2015
Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans.
Article in Archives of oral biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Multilocus sequence typing and phenotypic properties of Streptococcus mutans from Thai children with different caries statuses.BMC oral health · 2024Article
- Intraspecies interactions ofmSphere · 2024Article
- Intraspecies interactions ofbioRxiv : the preprint server for biology · 2023Article
- [Association of sepM gene mutation with mutacin Ⅳ production byNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Article
- Transmission patterns of Streptococcus mutans demonstrated by a combined rep-PCR and MLST approach.Clinical oral investigations · 2018Article
- Assessment of MultiLocus Sequence Analysis As a Valuable Tool for the Classification of the GenusFrontiers in microbiology · 2017Article
- Relationship between Pyruvate Kinase Activity and Cariogenic Biofilm Formation inFrontiers in microbiology · 2017Article
- Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.Journal of microbiological methods · 2016Article
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5 authors.
Funding
Abstract
objectiveTwo multilocus sequencing typing (MLST) schemes are currently available for Streptococcus mutans. The first, introduced by Nakano et al. in 2007, consists of 8 conserved housekeeping genes. The second, introduced in 2010 by Do et al., includes 6 housekeeping genes and 2 putative virulence genes. The purpose of the current study was to compare the two MLST schemes for use in validating repetitive extragenic palindromic polymerase chain reaction (rep-PCR) genotypes.
designThirty-three S. mutans isolates, representing the 11 most commonly occurring rep-PCR genotype groups, were selected for MLST. MLST was performed with SYBR Green™ PCR with published primers for both MLST schemes. Amplicons were purified, sequenced, and data checked against the www.PubMLST.org database for allelic and sequence type (ST) assignment. Discriminatory power, congruence, and convenience criteria were evaluated. Concatenated sequences for each scheme were analyzed using MEGA to generate phylogenetic trees using minimum evolution with bootstrap.
resultsNo significant difference in discriminatory power was observed between the two MLST schemes for S. mutans. Clonal clusters were consistent for both schemes. Overall, MLST demonstrated marginally greater discriminatory power than rep-PCR; however all methods were found to be congruent. New alleles and ST are reported for each scheme and added to the PubMLST database.
conclusionsClonality, supported by both methods and rep-PCR, indicates S. mutans genotypes are shared between unrelated subjects. Both Nakano and Do schemes demonstrates similar genotype discrimination for S. mutans isolates suggesting each are well designed and may be used to verify rep-PCR genotypes.
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