Evidence map›Paper›PMID 26438964›Full record

ArticleACS applied materials & interfaces2015

Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor.

Frankie J Rawson, Jacqueline Hicks, Nicholas Dodd, Wondwossen Abate, David J Garrett, Nga Yip, Gyorgy Fejer, Alison J Downard, Kim H R Baronian, Simon K Jackson and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied materials & interfaces, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 30 citations in OpenAlex.

  1. High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Frankie J RawsonLaboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham , University Park, Nottingham NG7 2RD, United Kingdom.
Jacqueline HicksLaboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham , University Park, Nottingham NG7 2RD, United Kingdom.
Nicholas DoddCentre for Biomedical Research, School of Biomedical and Healthcare Science, Plymouth University , Drake Circus, Plymouth, Devon PL4 8AA, United Kingdom.
Wondwossen AbateCentre for Biomedical Research, School of Biomedical and Healthcare Science, Plymouth University , Drake Circus, Plymouth, Devon PL4 8AA, United Kingdom.
David J GarrettSchool of Physics, The University of Melbourne , Victoria 3010, Australia.
Nga YipSchool of Chemical Engineering, University of Birmingham , Edgbaston, Birmingham B15 2TT, United Kingdom.
Gyorgy FejerCentre for Biomedical Research, School of Biomedical and Healthcare Science, Plymouth University , Drake Circus, Plymouth, Devon PL4 8AA, United Kingdom.
Alison J Downard
Kim H R Baronian
Simon K JacksonCentre for Biomedical Research, School of Biomedical and Healthcare Science, Plymouth University , Drake Circus, Plymouth, Devon PL4 8AA, United Kingdom.
Paula M MendesSchool of Chemical Engineering, University of Birmingham , Edgbaston, Birmingham B15 2TT, United Kingdom.
University of Plymouth · GBUniversity of Birmingham · GBUniversity of Nottingham · GBUniversity of Melbourne · AU

Funding

National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/L00058X/1
6 · The paper itself

Abstract

Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular "pulse" of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.

Indexed as

Biosensing TechniquesAnimalsLipopolysaccharidesMacrophagesMiceNADPH OxidasesNanotubes, CarbonReactive Oxygen SpeciesToll-Like Receptor 4LipopolysaccharidesNADPH OxidasesNanotubes, CarbonReactive Oxygen SpeciesTlr4 protein, mouseToll-Like Receptor 4carbon nanotubeselectrocatalytic sensorhydrogen peroxideintracellular sensormacrophage cellsreactive oxygen species

Identifiers

PMID26438964
PMCPMC4654508
OpenAlexW2243993274

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.