ArticleThe Journal of biological chemistry2015
Multipart Chaperone-Effector Recognition in the Type III Secretion System of Chlamydia trachomatis.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Insights into the association of the Chlamydia trachomatis type III secretion chaperone complex, Scc4:Scc1, from sequential expression in Escherichia coli.Protein expression and purification · 2024Article
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- Diversity of σJournal of bacteriology · 2023Article
- Genome copy number regulates inclusion expansion, septation, and infectious developmental form conversion inJournal of bacteriology · 2021Article
- Chain-Selective Isotopic Labeling of the Heterodimeric Type III Secretion Chaperone, Scc4:Scc1, Reveals the Total Structural Rearrangement of theBiomolecules · 2020Article
- Backbone and sidechain resonance assignments and secondary structure of Scc4 from Chlamydia trachomatis.Biomolecular NMR assignments · 2020Article
- Context-Dependent Action of Scc4 Reinforces Control of the Type III Secretion System.Journal of bacteriology · 2020Article
- Purification of Tag-FreeBiochemistry · 2019Article
- Characterization of lasR-deficient clinical isolates of Pseudomonas aeruginosa.Scientific reports · 2018Article
- Potency of Solithromycin against Fast- and Slow-Growing Chlamydial Organisms.Antimicrobial agents and chemotherapy · 2018Article
- Article
- Chlamydia cell biology and pathogenesis.Nature reviews. Microbiology · 2016Review
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- MxiA, MxiC and IpaD Regulate Substrate Selection and Secretion Mode in the T3SS of Shigella flexneri.PloS one · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Secretion of effector proteins into the eukaryotic host cell is required for Chlamydia trachomatis virulence. In the infection process, Scc1 and Scc4, two chaperones of the type III secretion (T3S) system, facilitate secretion of the important effector and plug protein, CopN, but little is known about the details of this event. Here we use biochemistry, mass spectrometry, nuclear magnetic resonance spectroscopy, and genetic analyses to characterize this trimolecular event. We find that Scc4 complexes with Scc1 and CopN in situ at the late developmental cycle of C. trachomatis. We show that Scc4 and Scc1 undergo dynamic interactions as part of the unique bacterial developmental cycle. Using alanine substitutions, we identify several amino acid residues in Scc4 that are critical for the Scc4-Scc1 interaction, which is required for forming the Scc4·Scc1·CopN ternary complex. These results, combined with our previous findings that Scc4 plays a role in transcription (Rao, X., Deighan, P., Hua, Z., Hu, X., Wang, J., Luo, M., Wang, J., Liang, Y., Zhong, G., Hochschild, A., and Shen, L. (2009) Genes Dev. 23, 1818-1829), reveal that the T3S process is linked to bacterial transcriptional events, all of which are mediated by Scc4 and its interacting proteins. A model describing how the T3S process may affect gene expression is proposed.
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